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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Overview
Product name
Anti-Blooms Syndrome Protein Blm (phospho T99) antibody
See all Blooms Syndrome Protein Blm products (8) ...
Description
Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)
Specificity
ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99.
Tested applications
WB, IHC-P, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic phosphopeptide derived from human Bloom Syndrome Protein Blm around the phosphorylation site of threonine 99 (Q-E-TP-Q-R).
Positive control
HeLa,HepG2 cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
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Immunocytochemistry/ Immunofluorescence - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
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Western blot - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab62206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 159 kDa.
IHC-P: 1/50 - 1/100.
ICC/IF: 1/100 - 1/500.
ELISA
ELISA: 1/5000ELISA: 1/5000
Target
Function
Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.
Involvement in disease
Defects in BLM are the cause of Bloom syndrome (BLM) [MIM:210900]. BLM is an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability.
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HRDC domain.
Post-translational
modifications
Phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP54132
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RecQ-like type 2 antibody
- BLM antibody
- BLM_HUMAN antibody
see all
Database links
- Entrez Gene: 641 Human
- Omim: 604610 Human
- SwissProt: P54132 Human
- Unigene: 169348 Human
- Unigene: 725208 Human
Anti-Blooms Syndrome Protein Blm (phospho T99) antibody images:
Immunocytochemistry/ Immunofluorescence - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Immunofluorescence analysis of HeLa cells using Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206) at 1/500 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
ab62206 staining Blooms Syndrome Protein Blm (phospho T99) in Human heart by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The picture on the right is treated with the synthesized peptide.
Western blot - Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
All lanes : Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate with blocking peptide
Predicted band size : 159 kDa
Observed band size : 159 kDa
References for Anti-Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)
ab62206 has not yet been referenced specifically in any publications.
Publishing research using ab62206? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"