Blooms Syndrome Protein Blm (phospho T99) antibody (ab62206)

Product Name 

Blooms Syndrome Protein Blm (phospho T99) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Blooms Syndrome Protein Blm (phospho T99)

Immunogen 

Synthetic phosphopeptide derived from human Bloom Syndrome Protein Blm around the phosphorylation site of threonine 99 (Q-E-T^P-Q-R).

Reacts with 
(species key)

Hu, Ms

Specificity 

ab62206 detects endogenous levels of Bloom Syndrome Protein Blm only when phosphorylated at threonine 99.

Tested applications 
(see key)

ELISA, ICC/IF

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cells

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.

Relevance 

The Bloom's syndrome (BS) gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DExH box-containing DNA and RNA helicases. The BLM protein has similarity to 2 other proteins that are members of the subfamily, namely the gene product encoded by RECQL2, also called the Werner syndrome gene (WRN), and the product of the yeast gene SGS1. These proteins may interact with topoisomerases, have 42 to 44% amino acid identity across the conserved helicase motifs, are of similar length and contain highly negatively charged N-terminal regions and highly positively charged C-terminal regions.

The BLM protein is located in the nucleus of normal human cells in the nuclear domain 10 (ND10) or promyelocytic leukemia nuclear (PML) bodies. These structures are punctate deposits of proteins disrupted upon viral infection and in certain human malignancies. BLM was found primarily in ND10 except during S phase, when it colocalized with the Werner syndrome gene product, WRN, in the nucleolus. The BLM protein is likely to be part of a DNA surveillance mechanism operating during S phase - BLM was found to be part of the BASC (BRCA1-associated genome surveillance) complex, which may serve as a sensor of abnormal DNA structures and/or as a regulator of the postreplication repair process.

Bloom syndrome cells show marked genomic instability; in particular, hyperrecombination between sister chromatids and homologous chromosomes - SCE (sister chromatid exchanges). In vitro BLM selectively binds Holliday junctions formed during genetic recombination and acts on recombination intermediates containing a Holliday junction to promote ATP-dependent branch migration. BLM may disrupt potentially recombinogenic molecules that arise at sites of stalled replication forks.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab62206 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

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