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ab14389 has been referenced in 16 publications.
Publishing research using ab14389? Please let us know so that we can cite the reference in this datasheet
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SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.55 Triton-X100 and incubated for 1 hour with ab14389 (1/200). The Bmi1 staining is shown in red. The cells were counterstained with DAPI (blue). 100x magnification.
Darin McDonald, Cross Cancer Institute
ab14389 staining Bmi1 in human head and neck squamous cell cancers (HNSCC) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Sections were deparaffinized in xylene, dehydrated through graded alcohols, and placed in 0.1% hydrogen peroxide to quench any endogenous peroxidase activity. A 5 minute, 750 W microwave pretreatment in citrate buffer (pH 6.0) was repeated 4 times and followed by treatment with 10% normal rabbit serum for 30 minutes to block nonspecific antibody binding. The slides were then incubated with ab14389 at a 1/100. Primary antibody incubation was followed by three washes with PBST and incubation with fluorescently-labeled Cy2 (1/250) secondary antibody for 2 hours. Nuclei were counterstained with DAPI. Finally, sections were rinsed with PBST, dehydrated through sequential washes in 50%, 70%, 95%, and 100% ethanol and then cleared in xylene. Slides were mounted with DPX mounting media and allowed to dry overnight.
Image from Ganguli-Indra G et al, PLoS One. 2009;4(4):e5367. Epub 2009 Apr 28, Fig 2.
Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389) at 1/500 dilution + whole cell lysate prepared from Panc1 cancer cells at 35 µg
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 27 kDa,48 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 10 minutes
Image courtesy of an anonymous Abreview.
Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389) at 1/500 dilution + whole tissue lysate prepared from murine pancreatic cancer at 50 µg
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 15 minutes
Image courtesy of an anonymous Abreview.
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