Anti-Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)

  Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)

SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.55 Triton-X100 and incubated for 1 hour with ab14389 (1/200). The Bmi1 staining is shown in red. The cells were counterstained with DAPI (blue). 100x magnification.

Darin McDonald, Cross Cancer Institute

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)

ab14389 staining Bmi1 in human head and neck squamous cell cancers (HNSCC) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Sections were deparaffinized in xylene, dehydrated through graded alcohols, and placed in 0.1% hydrogen peroxide to quench any endogenous peroxidase activity. A 5 minute, 750 W microwave pretreatment in citrate buffer (pH 6.0) was repeated 4 times and followed by treatment with 10% normal rabbit serum for 30 minutes to block nonspecific antibody binding. The slides were then incubated with ab14389 at a 1/100. Primary antibody incubation was followed by three washes with PBST and incubation with fluorescently-labeled Cy2 (1/250) secondary antibody for 2 hours. Nuclei were counterstained with DAPI. Finally, sections were rinsed with PBST, dehydrated through sequential washes in 50%, 70%, 95%, and 100% ethanol and then cleared in xylene. Slides were mounted with DPX mounting media and allowed to dry overnight.

Image from Ganguli-Indra G et al, PLoS One. 2009;4(4):e5367. Epub 2009 Apr 28, Fig 2.