Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)

Product Name 

Bmi1 antibody [1.T.21] - ChIP Grade

Product type 

Primary antibodies

Description 

Mouse monoclonal [1.T.21] to Bmi1 - ChIP Grade

Immunogen 

Recombinant fragment, corresponding to amino acids 1-202 of Mouse Bmi1

Reacts with 
(species key)

Hu, Ms, Rat, Rb, Zfsh

Specificity 

Recognizes mouse Bmi-1(triplet).

Tested applications 
(see key)

ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18332116).
Flow Cyt: Use at a concentration of 0.65 µg/ml (PMID 19001505).
ICC: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 0.65 µg/ml (PMID 19001505).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.2 - 2.0 µg/ml. Predicted molecular weight: 37 kDa.
Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab14389 0.2ug/ml. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
IHC-P: 1/100 (from PubMed:16936260).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

U2OS whole cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation

Relevance 

The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.

Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

1.T.21

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.15M Tris-glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 0.50 - 1.00 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for Bmi1 antibody [1.T.21] - ChIP Grade images, references, products related to ab14389 and other tools.

Bmi1 antibody [1.T.21] - ChIP Grade images:

References for Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)

  This product has been used in: (two most recent references)

Ganguli-Indra G et al. CTIP2 expression in human head and neck squamous cell carcinoma is linked to poorly differentiated tumor status. PLoS ONE 4:e5367 (2009). IHC-P; Human. PubMed: 19399189

Boukarabila H et al. The PRC1 Polycomb group complex interacts with PLZF/RARA to mediate leukemic transformation. Genes Dev 23:1195-206 (2009). ChIP; Human. PubMed: 19451220

See all 9 publication references for this product.


If you publish research using ab14389 please let us know so that we can cite the reference on this datasheet.

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