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Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)
![Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)](/ps/datasheet/images/ab14389_1.jpg)
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Product Name
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Bmi1 antibody [1.T.21] - ChIP Grade
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See all Bmi1 antibodies (8)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [1.T.21] to Bmi1 - ChIP Grade
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Immunogen
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Recombinant fragment, corresponding to amino acids 1-202 of Mouse Bmi1
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Reacts with
(species key)
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Hu, Ms, Rat, Rb, Zfsh
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Specificity
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Recognizes mouse Bmi-1(triplet).
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Tested applications
(see key)
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ChIP, Flow Cyt, ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 18332116).
Flow Cyt: Use at a concentration of 0.65 µg/ml (PMID 19001505).
ICC: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 0.65 µg/ml (PMID 19001505).
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.2 - 2.0 µg/ml. Predicted molecular weight: 37 kDa.
Detects Bmi-1 in RIPA lysates from U2OS cells. U2OS cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab14389 0.2ug/ml. Proteins were visualized using a goat anti-mouse IgG labeled with HRP and a chemiluminescence detection system.
IHC-P: 1/100 (from PubMed:16936260).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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U2OS whole cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation
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Relevance
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The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.
Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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1.T.21
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium Chloride, 0.15M Tris-glycine. pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bmi1 antibody [1.T.21] - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bmi1 antibody [1.T.21] - ChIP Grade images, references, products related to ab14389 and other tools.
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Bmi1 antibody [1.T.21] - ChIP Grade images:
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 Immunocytochemistry - Bmi1 antibody [1.T.21] - Hematopoietic Stem Cell Marker (ab14389)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.55 Triton-X100 and incubated for 1 hour with ab14389 (1/200). The Bmi1 staining is shown in red. The cells were counterstained with DAPI (blue). 100x magnification. Darin McDonald, Cross Cancer Institute
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References for Bmi1 antibody [1.T.21] - ChIP Grade (ab14389)
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This product has been used in: (two most recent references)
Hyry V et al. Bmi-1 expression predicts prognosis in squamous cell carcinoma of the tongue. Br J Cancer : (2010). IHC-P; Human. PubMed: 20145620
Ganguli-Indra G et al. CTIP2 expression in human head and neck squamous cell carcinoma is linked to poorly differentiated tumor status. PLoS ONE 4:e5367 (2009). IHC-P; Human. PubMed: 19399189
See all 10 publication references for this product.
If you publish research using ab14389 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Bmi1
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Bmi1 antibody [1.T.21] - ChIP Grade - more information
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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