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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab25791? Please let us know so that we can cite the reference in this datasheet
ab25791 has been referenced in 1 publications.
- Kim SY et al. Juxtaposed Polycomb complexes co-regulate vertebral identity. Development 133:4957-68 (2006). PubMed: 17107999
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Bmi1 antibody (ab25791)
Anti-Bmi1 antibody (ab25791) at 1/1000 dilution + U2OS whole cell lysate at 20 µg
Secondary
Rabbit anti-Goat IgG [H&L] at 1/20000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
The membrane was blocked in PBS containing 5% nonfat dry milk then probed overnight at 4°C with ab25791.
Immunocytochemistry/ Immunofluorescence - Bmi1 antibody (ab25791)
ICC/IF image of ab25791 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25791, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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