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Anti-Bmi1 antibody (ab25791)

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1 question for ab25791

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Question 1

Thursday 17-August-2006

I'm working on Psc and Su(z)2, which are the Drosophila homologues of Bmi-1. I would be interested in knowing whether any of your Bmi1 antibodies (ab25791, ab38295, and ab14389) cross react with either of the Drosophila homologues. Would it be possible for me to test a 1:1,000 dilution of each of the antibodies on WBs for cross- reactivity? Alternatively, I could send you a blot to test for yourself. I'm happy to buy any ab's that cross-react, but I can't justify spending $300/ab without knowing whether any of them will cross-react...

ANSWER:

  

Thank you for your enquiry. I performed a homology search of the immunogen of ab25791 and ab14399 and of the whole human protein with the drosophila protein and unfortunately found that it is so low that it is not detected by a BLAST search. I therefore do not think those antibodies will detect the Drosophila protein and recommend to purchase Drosophila specific antibodies.

I'm sorry we currently do not have those in our catalogue and wish you all the best in your research,

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