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Products:Cell Biology >> Cell Cycle >> Cell differentiation
Overview
Product name
Anti-Bmi1 antibody
See all Bmi1 products (10) ...
Description
Goat polyclonal to Bmi1
Tested applications
ICC/IF, IF, WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cat, Dog
Immunogen
Synthetic peptide: CDSGSDKANSPAGG, corresponding to amino acids 252-264 of Human Bmi1
CDSGSDKANS PAGG
Positive control
U2OS whole cell lysate
General notes
This antibody replaces ab3833.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IF: Use at an assay dependent dilution.
WB: 1/500 - 1/3000.Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA
ELISA: 1/5000 - 1/30000.ELISA: 1/5000 - 1/30000.
Target
Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Sequence similarities
Contains 1 RING-type zinc finger.
Post-translational
modifications
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionP35226
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B lymphoma Mo MLV insertion region (mouse) antibody
- Bmi 1 antibody
- BMI1 antibody
see all
Database links
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 307151 Rat
- Omim: 164831 Human
- SwissProt: Q9TST0 Cat
- SwissProt: P35226 Human
- SwissProt: P25916 Mouse
see all
Anti-Bmi1 antibody images:
Western blot - Bmi1 antibody (ab25791)
Anti-Bmi1 antibody (ab25791) at 1/1000 dilution + U2OS whole cell lysate at 20 µg
Secondary
Rabbit anti-Goat IgG [H&L] at 1/20000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
The membrane was blocked in PBS containing 5% nonfat dry milk then probed overnight at 4°C with ab25791.
Immunocytochemistry/ Immunofluorescence - Bmi1 antibody (ab25791)
ICC/IF image of ab25791 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25791, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Bmi1 antibody (ab25791)
This product has been referenced in:
- Kim SYet al. Juxtaposed Polycomb complexes co-regulate vertebral identity. Development 133:4957-68 (2006).Read more (PubMed: 17107999) »
See 1 publication for this product
Publishing research using ab25791? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"