Overview

  • Product nameAnti-Bmi1 antibody
    See all Bmi1 primary antibodies
  • Description
    Goat polyclonal to Bmi1
  • Tested applicationsICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog
  • Immunogen

    Synthetic peptide:

    CDSGSDKANSPAGG

    , corresponding to amino acids 252-264 of Human Bmi1

  • Positive control
    • U2OS whole cell lysate
  • General notesThis antibody replaces ab3833.

Properties

Applications

Our Abpromise guarantee covers the use of ab25791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA 1/5000 - 1/30000.

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    • RNF51 antibody
    see all

Anti-Bmi1 antibody images

  • Anti-Bmi1 antibody (ab25791) at 1/1000 dilution + U2OS whole cell lysate at 20 µg

    Secondary
    Rabbit anti-Goat IgG [H&L] at 1/20000 dilution

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
  • ICC/IF image of ab25791 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25791, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Bmi1 antibody (ab25791)

This product has been referenced in:

See 1 Publication for this product

Product Wall

The cysteine added to the end of an immunizing peptide is to couple the peptide to its carrier protein to create immune response. This method is a favorable method for specific antibody formation and allows defined orientation of the antigen and minimi...

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Thank you for your enquiry. I performed a homology search of the immunogen of ab25791 and ab14399 and of the whole human protein with the drosophila protein and unfortunately found that it is so low that it is not detected by a BLAST search. I theref...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"