Anti-Bmi1 antibody (ab25791)

Overview

• Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
• Description
  Goat polyclonal to Bmi1
• Tested applicationsICC/IF, WB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cat, Dog
• Immunogen

  Synthetic peptide: CDSGSDKANSPAGG, corresponding to amino acids 252-264 of Human Bmi1

  Run BLAST with Run BLAST with
• Positive controlU2OS whole cell lysate
• General notesThis antibody replaces ab3833.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Other

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
• Sequence similaritiesContains 1 RING-type zinc finger.
• Post-translational
  modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession P35226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 554342 Cat
  • Entrez Gene: 487097 Dog
  • Entrez Gene: 100532731 Human
  • Entrez Gene: 648 Human
  • Entrez Gene: 12151 Mouse
  • Entrez Gene: 307151 Rat
  • Omim: 164831 Human
  • SwissProt: Q9TST0 Cat

  • SwissProt: P35226 Human
  • SwissProt: P25916 Mouse
  • Unigene: 380403 Human
  • Unigene: 289584 Mouse

  see all

• Alternative names
  B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody

  BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibodyRNF51 antibody

  see all

Anti-Bmi1 antibody images

• 

  Western blot - Bmi1 antibody (ab25791)
  Anti-Bmi1 antibody (ab25791) at 1/1000 dilution + U2OS whole cell lysate at 20 µg
  
  Secondary
  Rabbit anti-Goat IgG [H&L] at 1/20000 dilution
  
  Predicted band size : 37 kDa
  Observed band size : 37 kDa
  
• 

  Immunocytochemistry/ Immunofluorescence - Bmi1 antibody (ab25791)
  ICC/IF image of ab25791 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25791, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.