Anti-Bmi1 antibody (ab38295)
- Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
- DescriptionRabbit polyclonal to Bmi1
- Tested applicationsICC/IF, IHC-P, WB, IHC-Fr, IP more details
- Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
- Positive control
- K562 cell lysate.
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
Our Abpromise guarantee covers the use of ab38295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use a concentration of 10 µg/ml.|
|IHC-P||IHC-P: Use at an assay dependent concentration. PubMed: 20083111|
|WB||WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).|
- FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
- Sequence similaritiesContains 1 RING-type zinc finger.
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
- Cellular localizationNucleus. Cytoplasm.
- B lymphoma Mo MLV insertion region (mouse) antibody
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- Bmi 1 antibody
- BMI1 antibody
- BMI1 polycomb ring finger oncogene antibody
- BMI1_HUMAN antibody
- Flvi 2/bmi 1 antibody
- FLVI2/BMI1 antibody
- MGC12685 antibody
- Murine leukemia viral (bmi 1) oncogene homolog antibody
- Oncogene BMI 1 antibody
- PCGF 4 antibody
- PCGF4 antibody
- Polycomb complex protein BMI 1 antibody
- Polycomb complex protein BMI-1 antibody
- Polycomb group protein Bmi1 antibody
- Polycomb group ring finger 4 antibody
- Polycomb group RING finger protein 4 antibody
- RING finger protein 51 antibody
- RNF 51 antibody
- RNF51 antibody
Anti-Bmi1 antibody images
ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate
Predicted band size : 37 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
References for Anti-Bmi1 antibody (ab38295)
This product has been referenced in:
- Szabo AZ et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). Read more (PubMed: 23335266) »
- Xi S et al. Cigarette smoke mediates epigenetic repression of miR-487b during pulmonary carcinogenesis. J Clin Invest 123:1241-61 (2013). ChIP ; Human . Read more (PubMed: 23426183) »