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Anti-Bmi1 antibody (ab38295)

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Overview

Product name

Anti-Bmi1 antibody
See all Bmi1 products (10) ...

Description

Rabbit polyclonal to Bmi1

Tested applications

ICC/IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

Positive control

K562 cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation

  • Western blot - Bmi1 antibody (ab38295)Western blot - Bmi1 antibody (ab38295) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Sequence similarities

Contains 1 RING-type zinc finger.

Post-translational
modifications

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Cellular localization

Nucleus. Cytoplasm.

Target information above from: UniProt accessionP35226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • B lymphoma Mo MLV insertion region (mouse) antibody
  • Bmi 1 antibody
  • BMI1 antibody
  • BMI1 polycomb ring finger oncogene antibody
  • BMI1_HUMAN antibody
  • Flvi 2/bmi 1 antibody
  • FLVI2/BMI1 antibody
  • MGC12685 antibody
  • Murine leukemia viral (bmi 1) oncogene homolog antibody
  • Oncogene BMI 1 antibody
  • PCGF 4 antibody
  • PCGF4 antibody
  • Polycomb complex protein BMI 1 antibody
  • Polycomb complex protein BMI-1 antibody
  • Polycomb group protein Bmi1 antibody
  • Polycomb group ring finger 4 antibody
  • Polycomb group RING finger protein 4 antibody
  • RING finger protein 51 antibody
  • RNF 51 antibody
  • RNF51 antibody
see all

Database links

see all

Anti-Bmi1 antibody images:

  Western blot - Bmi1 antibody (ab38295)

Western blot - Bmi1 antibody (ab38295)

Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate


Predicted band size : 37 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)

References for Anti-Bmi1 antibody (ab38295)

This product has been referenced in:

  • Toribio REet al. The midregion, nuclear localization sequence, and C terminus of PTHrP regulate skeletal development, hematopoiesis, and survival in mice. FASEB J : (2010). WB; Mouse.Read more (PubMed: 20145205) »
  • Tokar EJet al. Arsenic-specific stem cell selection during malignant transformation. J Natl Cancer Inst 102:638-49 (2010). ICC/IF; Human.Read more (PubMed: 20339138) »

See all 4 publications for this product

Publishing research using ab38295? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"