Overview
- Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
- DescriptionRabbit polyclonal to Bmi1
- Tested applicationsICC/IF, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
- Positive controlK562 cell lysate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab38295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
| IHC-P | IHC-P: Use at an assay dependent dilution. PubMed: 20083111 |
| WB | WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Bmi1 peptide (ab39733). |
Target
- FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
- Sequence similaritiesContains 1 RING-type zinc finger.
- Post-translational
modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. - Cellular localizationNucleus. Cytoplasm.
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Database links
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Entrez Gene: 307151 Rat
- Omim: 164831 Human
- SwissProt: P35226 Human
- SwissProt: P25916 Mouse
- Unigene: 380403 Human
- Unigene: 289584 Mouse
see all
Target information above from: UniProt accession
P35226
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody
- BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibody
see all
Anti-Bmi1 antibody images
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Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate
Predicted band size : 37 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?) -
Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
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Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.
References for Anti-Bmi1 antibody (ab38295)
This product has been referenced in:
- Szabo AZ et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). Read more (PubMed: 23335266) »
- Dong P et al. Mutant p53 gain-of-function induces epithelial-mesenchymal transition through modulation of the miR-130b-ZEB1 axis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22847613) »


