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Overview

  • Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
  • Description
    Rabbit polyclonal to Bmi1
  • Tested applicationsICC/IF, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

  • Positive controlK562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use a concentration of 10 µg/ml.
IHC-P IHC-P: Use at an assay dependent dilution. PubMed: 20083111
WB WB: Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Bmi1 peptide (ab39733).

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.

    • Target information above from: UniProt accession P35226 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody
      BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibody
    see all

Anti-Bmi1 antibody images

  • Western blot - Bmi1 antibody (ab38295)
    Western blot - Bmi1 antibody (ab38295)
    Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
    Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
    Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : K562 cell lysate


    Predicted band size : 37 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/ Immunofluorescence-Anti-Bmi1 antibody(ab38295)
    Immunocytochemistry/ Immunofluorescence-Anti-Bmi1 antibody(ab38295)
    Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

  • Immunocytochemistry/ Immunofluorescence-Anti-Bmi1 antibody(ab38295)
    Immunocytochemistry/ Immunofluorescence-Anti-Bmi1 antibody(ab38295)
    Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

References for Anti-Bmi1 antibody (ab38295)

This product has been referenced in:
  • Szabo AZ  et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). Read more (PubMed: 23335266) »
  • Dong P  et al. Mutant p53 gain-of-function induces epithelial-mesenchymal transition through modulation of the miR-130b-ZEB1 axis. Oncogene : (2012). WB ; Human . Read more (PubMed: 22847613) »

See all 6 Publications for this product

Publishing research using ab38295 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab38295 Submit a Question for ab38295

Displaying 1 - 8 of 8 results for Abreviews and Q&A

10
Votes: Highest First

Western blot abreview for Anti-Bmi1 antibody

 Good
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (MKN45)
Loading amount 30 µg
Specification MKN45
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Username

Verified customer

Submitted May 14 2013

I read your data sheet of the Bmi1 ab again and found that the
predicted molecular weight in the data sheet (but also from Genecard) is
about 37 kDa. Since the protein is probably glycosylated, it may even be
bigger. The WB on your datafile...

Read More

Thank you for your response.
I have discussed this enquiry with my colleagues.
A BLAST with the immunogen in the human proteome does not predict cross-reactivity with any other proteins, just BMI-1 and partial BMI-1 sequences. It could be a clea...

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Please find the questionnaire attached. In addition I add the picture of
a staining.
Best
IHC Questionnaire
It will take approximately 5 minutes to complete the questionnaire.
Please fill-in all fields so that we can better assist...

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Thank you for your enquiry regarding ab38295 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
My colleague has asked me to look after her customers whi...

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Sehr geehrte Damen und Herren, wir benutzen zur Darstellung Bmi1
positiver Zellen im Darm ihren Ak ab38295. Dies erfolgt an
Paraffinblöcken nach retrieval. Die Färbung erscheint soweit ganz in
Ordnung, allerdings ist die Verteilun...

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Vielen Dank für Ihre Anfrage.
Es tut mir Leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Laut unseren Unterlagen haben wir den Antikörper nicht selber in der IHC getestet, sondern haben diese Applikation aufgrund einer Publikation aufge...

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shipment was sent out monday and received tuesday. nonetheless customer reported box was warm inside and out when received. all 4 antibodies are reported as not working from original shipment on order 1105213. Tested older vials of ab13533 and ab38295 and...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the order...

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Dear tech,

Please check below reply from the customer .Thank you!

1.) Can you confirm that the bmi1 protein is present in theMHCC97-H cellline? (Is there for example a publication describing this?)

Yes, I have c...

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Thank you very much for following this up with the customer. I am sorry that the change in the blocking buffer did not improve the results.

I would still like to recommend to the customer to reduce the samples for the Western blot either with DT...

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1) Abcam product code ab38295

2) Abcam order reference number or product batch number


3) Storage conditions:Store at +4°C.

4) Description of the problem (high background, no band, wrong band size...)

n...

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Thank you for taking time to liaise with the customer and to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide ...

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I'm working on Psc and Su(z)2, which are the Drosophila homologues of Bmi-1. I would be interested in knowing whether any of your Bmi1 antibodies (ab25791, ab38295, and ab14389) cross react with either of the Drosophila homologues. Would it be poss...

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Thank you for your enquiry. I performed a homology search of the immunogen of ab25791 and ab14399 and of the whole human protein with the drosophila protein and unfortunately found that it is so low that it is not detected by a BLAST search. I therefore...

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