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Western blot - Bmi1 antibody (ab38295)
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Product Name
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Bmi1 antibody
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See all Bmi1 antibodies (9)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bmi1
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Immunogen
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Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa. Can be blocked with Bmi1 peptide (ab39733).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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K562 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation
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Relevance
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The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.
Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bmi1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bmi1 antibody images, references, products related to ab38295 and other tools.
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Bmi1 antibody images:
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 Western blot - Bmi1 antibody (ab38295)
Lane 1 : Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Bmi1 antibody (ab38295) at 2 µg/ml
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate
Predicted band size : 37 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to Bmi1
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Bmi1 antibody - more information
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Customer reviews (feedback) regarding Bmi1 antibody |
Customer FAQs regarding Bmi1 antibody |
Protocols for Bmi1 antibody |
Price and availability of products related to Bmi1 antibody |
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Bmi1 antibody - related products:
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Bmi1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bmi1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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