Feedback Form

Western blot - Bmi1 antibody (ab38295)
Western blot - Bmi1 antibody (ab38295)
(enlarge)


Product Name 

Bmi1 antibody

 

See all Bmi1 antibodies (9)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Bmi1

Immunogen 

Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews

Submit an Abreview

Be the first to review this antibody and earn 220 Abpoints.


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa. Can be blocked with Bmi1 peptide (ab39733).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

K562 cell lysate.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation

Relevance 

The Bmi1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.

Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.

 

Alternative names Bmi1 antibody (ab38295)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

648    (Human)
12151    (Mouse)
307151    (Rat)

GeneCard

    

GC10P022651    (Human)

Omim

    

164831    (Human)

SwissProt

    

P35226    (Human)
P25916    (Mouse)

Unigene

    

380403    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bmi1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Bmi1 antibody images, references, products related to ab38295 and other tools.

 

Bmi1 antibody images:

Product image  Western blot - Bmi1 antibody (ab38295)

Western blot - Bmi1 antibody (ab38295)

Lane 1 : Bmi1 antibody (ab38295) at 0.5 µg/ml
Lane 2 : Bmi1 antibody (ab38295) at 1 µg/ml
Lane 3 : Bmi1 antibody (ab38295) at 2 µg/ml

Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : K562 cell lysate


Predicted band size : 37 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to Bmi1

 

Bmi1 antibody - more information

Customer reviews (feedback) regarding Bmi1 antibody

Customer FAQs regarding Bmi1 antibody

Protocols for Bmi1 antibody

Price and availability of products related to Bmi1 antibody

 

Bmi1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Control Peptide (Blocking) for Bmi1 antibody

ab39733

  

Bmi1 peptide

 

Compatible Secondaries for Bmi1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Bmi1 Antibodies

See all Bmi1 antibodies (9)...

 

Bmi1 antibody - other tools:

Contact Abcam with a Technical Enquiry about Bmi1 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.