Overview

  • Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
  • Description
    Rabbit polyclonal to Bmi1
  • Tested applicationsICC/IF, IHC-P, WB, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 15 amino acids near the center of human Bmi1.

  • Positive control
    • K562 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab38295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 20083111
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 37 kDa.Can be blocked with Human Bmi1 peptide (ab39733).
IHC-Fr 1/300.
IP 1/2000.

Target

  • FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
  • Sequence similaritiesContains 1 RING-type zinc finger.
  • Post-translational
    modifications
    Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphoma Mo MLV insertion region (mouse) antibody
    • B lymphoma Mo MLV insertion region 1 homolog antibody
    • Bmi 1 antibody
    • BMI1 antibody
    • BMI1 polycomb ring finger oncogene antibody
    • BMI1_HUMAN antibody
    • Flvi 2/bmi 1 antibody
    • FLVI2/BMI1 antibody
    • MGC12685 antibody
    • Murine leukemia viral (bmi 1) oncogene homolog antibody
    • Oncogene BMI 1 antibody
    • PCGF 4 antibody
    • PCGF4 antibody
    • Polycomb complex protein BMI 1 antibody
    • Polycomb complex protein BMI-1 antibody
    • Polycomb group protein Bmi1 antibody
    • Polycomb group ring finger 4 antibody
    • Polycomb group RING finger protein 4 antibody
    • RING finger protein 51 antibody
    • RNF 51 antibody
    • RNF51 antibody
    see all

Anti-Bmi1 antibody images

  • ab38295 staining Bmi1 in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/300) for 10 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab38295 staining Bmi1 in Rat fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 2.5% Goat serum for 10 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 2 hour at 25°C. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-Bmi1 antibody (ab38295) at 0.5 µg/ml
    Lane 2 : Anti-Bmi1 antibody (ab38295) at 1 µg/ml
    Lane 3 : Anti-Bmi1 antibody (ab38295) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : K562 cell lysate


    Predicted band size : 37 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of BMI-1 in K562 cells using ab38295 at 20 ug/ml.

References for Anti-Bmi1 antibody (ab38295)

This product has been referenced in:
  • Szabo AZ  et al. The CD44(+) ALDH(+) Population of Human Keratinocytes is Enriched for Epidermal Stem Cells with Long Term Repopulating Ability. Stem Cells : (2013). Read more (PubMed: 23335266) »
  • Dong P  et al. Identification of KLF17 as a novel epithelial to mesenchymal transition inducer via direct activation of TWIST1 in endometrioid endometrial cancer. Carcinogenesis N/A:N/A (2013). Read more (PubMed: 24220291) »

See all 8 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A/G
Sample Rat Cell lysate - whole cell (Oligodendrocytes)
Specification Oligodendrocytes
Total protein in input 30 µg
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Submitted Dec 09 2013

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (12.5%)
Sample Rat Cell lysate - whole cell (Oligodendrocyte)
Specification Oligodendrocyte
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 09 2013

Application Immunocytochemistry
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 2.5% · Temperature: 25°C
Sample Rat Cell (Fibroblast)
Specification Fibroblast
Permeabilization Yes - 0.01% triton X100
Fixative Paraformaldehyde
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Submitted Oct 30 2013

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Submitted Oct 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MKN45)
Loading amount 30 µg
Specification MKN45
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted May 14 2013

Thank you for your response.
I have discussed this enquiry with my colleagues.
A BLAST with the immunogen in the human proteome does not predict cross-reactivity with any other proteins, just BMI-1 and partial BMI-1 sequences. It could be a c...

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Thank you for your enquiry regarding ab38295 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
My colleague has asked me to look after her customers ...

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Vielen Dank für Ihre Anfrage.
Es tut mir Leid zu hören, dass Sie Probleme mit diesem Antikörper haben. Laut unseren Unterlagen haben wir den Antikörper nicht selber in der IHC getestet, sondern haben diese Applikation aufgrund einer Publikation au...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

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Thank you very much for following this up with the customer. I am sorry that the change in the blocking buffer did not improve the results.

I would still like to recommend to the customer to reduce the samples for the Western blot either with...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"