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Products:Cell Biology >> Cell Cycle >> Cell differentiation
Overview
Product name
Anti-Bmi1 antibody
See all Bmi1 products (10) ...
Description
Mouse monoclonal to Bmi1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-327 of Human Bmi1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Other
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC2
Cell Biology >> Cell Cycle >> Cell differentiation
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Western blot - Bmi1 antibody (ab54897)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Sequence similarities
Contains 1 RING-type zinc finger.
Post-translational
modifications
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
Cellular localization
Nucleus. Cytoplasm.
Target information above from: UniProt accessionP35226
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B lymphoma Mo MLV insertion region (mouse) antibody
- Bmi 1 antibody
- BMI1 antibody
see all
Database links
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Omim: 164831 Human
- SwissProt: P35226 Human
- Unigene: 380403 Human
Anti-Bmi1 antibody images:
Western blot - Bmi1 antibody (ab54897)
Bmi1 antibody (ab54897) at 1ug/lane + A-549 cell lysate at 25ug/lane.
References for Anti-Bmi1 antibody (ab54897)
This product has been referenced in:
- Jiang Yet al. Effect of siRNA-mediated silencing of Bmi-1 gene expression on HeLa cells. Cancer Sci 101:379-86 (2010).Read more (PubMed: 19930157) »
See 1 publication for this product
Publishing research using ab54897? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"