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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Bmi1.
(Peptide available as ab86660.)
Our Abpromise guarantee covers the use of ab63767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human Bmi1 peptide (ab86660). A more specific reaction is observed when 5% milk blocking is used, rather than BSA.|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
ab63767 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"