Anti-Bmi1 antibody (ab63767)

Overview

• Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
• Description
  Rabbit polyclonal to Bmi1
• Tested applicationsIHC-P, WB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rabbit, Horse, Cow, Cat, Dog, Pig, Chimpanzee
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Bmi1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab86660.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: MOLT4, U2OS, K562 It also gave a positive signal in FFPE human kidney carcinoma tissue sections.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
• Sequence similaritiesContains 1 RING-type zinc finger.
• Post-translational
  modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession P35226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 554342 Cat
  • Entrez Gene: 510666 Cow
  • Entrez Gene: 487097 Dog
  • Entrez Gene: 100532731 Human
  • Entrez Gene: 648 Human
  • Entrez Gene: 12151 Mouse
  • Omim: 164831 Human
  • SwissProt: Q9TST0 Cat

  • SwissProt: Q32KX7 Cow
  • SwissProt: P35226 Human
  • SwissProt: P25916 Mouse
  • Unigene: 380403 Human
  • Unigene: 289584 Mouse

  see all

• Alternative names
  B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody

  BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibody

  see all

Anti-Bmi1 antibody images

• 

  Western blot - Bmi1 antibody (ab63767)
  All lanes : Anti-Bmi1 antibody (ab63767) at 2 µg/ml (Blocked in 5% MILK)
  
  Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
  Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 37 kDa
  Observed band size : 37 kDa
  Additional bands at : 17 kDa. We are unsure as to the identity of these extra bands.
• 

  Western blot - Anti-Bmi1 antibody (ab63767)
  Anti-Bmi1 antibody (ab63767) at 2 µg/ml + Bmi1 protein (ab114169) at 0.1 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Exposure time : 30 seconds
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (ab63767)
  ICC/IF image of ab63767 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63767, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody (ab63767)
  IHC image of Bmi1 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63767, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.