Anti-Bmi1 antibody (ab85688)

Overview

• Product nameAnti-Bmi1 antibodySee all Bmi1 primary antibodies ...
• Description
  Rabbit polyclonal to Bmi1
• Tested applicationsICC/IF, WB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide, corresponding to a region between amino acids 276-326 of Human Bmi1 (NP_005171.4)

• Positive controlHeLa cells lysate, 293T cell lysate, NIH3T3 cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Hematopoietic Progenitors
  • Intracellular Molecules

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

Applications

Target

• FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
• Sequence similaritiesContains 1 RING-type zinc finger.
• Post-translational
  modificationsMonoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession P35226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100532731 Human
  • Entrez Gene: 648 Human
  • Entrez Gene: 12151 Mouse
  • Omim: 164831 Human
  • SwissProt: P35226 Human
  • SwissProt: P25916 Mouse
  • Unigene: 380403 Human
  • Unigene: 289584 Mouse

• Alternative names
  B lymphoma Mo MLV insertion region (mouse) antibodyB lymphoma Mo MLV insertion region 1 homolog antibodyBmi 1 antibody

  BMI1 antibodyBMI1 polycomb ring finger oncogene antibodyBMI1_HUMAN antibodyFlvi 2/bmi 1 antibodyFLVI2/BMI1 antibodyMGC12685 antibodyMurine leukemia viral (bmi 1) oncogene homolog antibodyOncogene BMI 1 antibodyPCGF 4 antibodyPCGF4 antibodyPolycomb complex protein BMI 1 antibodyPolycomb complex protein BMI-1 antibodyPolycomb group protein Bmi1 antibodyPolycomb group ring finger 4 antibodyPolycomb group RING finger protein 4 antibodyRING finger protein 51 antibodyRNF 51 antibodyRNF51 antibody

  see all

Anti-Bmi1 antibody images

• 

  Western blot - Bmi1 antibody (ab85688)
  All lanes : Anti-Bmi1 antibody (ab85688) at 0.1 µg/ml
  
  Lane 1 : HeLa cell lysate at 50 µg
  Lane 2 : HeLa cell lysate at 15 µg
  Lane 3 : HeLa cell lysate at 5 µg
  Lane 4 : 293T cell lysate at 50 µg
  Lane 5 : NIH3T3 cell lysate at 50 µg
  
  
  Predicted band size : 37 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 140 kDa,55 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunoprecipitation - Bmi1 antibody (ab85688)
  Detection of Human Bmi1 by mmunoprecipitation. Sample: HeLa Whole cell lysate (1 mg for IP, 20% of IP loaded). ab85688 at 3 ug/mg lysate was used for IP, and at 1ug/ml for subsequent WB detection.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Bmi1 antibody (ab85688)
  ICC/IF image of ab85688 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85688, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.