The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval in Sodium citrate pH6 buffer before commencing with IHC staining protocol.
Application notesIs unsuitable for WB.
FunctionComponent of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
(A) IHC staining for Bmi1 expression in primary Human neuroblastoma sample. A significant number of tumor cells show strong nuclear staining of Bmi1 (brown).
(B) Immunohistochemical staining of SCG and neuroblastoma samples for Bmi1.
References for Anti-Bmi1 antibody (ab97729)
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