Recombinant Anti-Bok antibody [EPR15331] (ab186745)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15331] to Bok
- Suitable for: WB, IP
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Bok antibody [EPR15331]
See all Bok primary antibodies -
Description
Rabbit monoclonal [EPR15331] to Bok -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal kidney and ovary cancer lysate; HeLa and PC-3 cell lysates; Rat spleen lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15331 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IP |
1/30 - 1/50.
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Notes |
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WB
1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IP
1/30 - 1/50. |
Target
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Function
May play a role in apoptosis. -
Tissue specificity
Expressed in brain, liver, appendix and lymphoid tissues. -
Sequence similarities
Belongs to the Bcl-2 family. - Information by UniProt
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Database links
- Entrez Gene: 666 Human
- Entrez Gene: 29884 Rat
- Omim: 605404 Human
- SwissProt: Q9UMX3 Human
- SwissProt: Q792S6 Rat
- Unigene: 293753 Human
- Unigene: 44461 Rat
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Alternative names
- Bcl 2 related ovarian killer protein antibody
- Bcl-2-like protein 9 antibody
- Bcl-2-related ovarian killer protein antibody
see all
Images
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All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human ovary cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
All lanes : Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
Anti-Bok antibody [EPR15331] (ab186745) at 1/1000 dilution + Rat spleen lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
Western blot analysis of Bok in PC-3 cell lysate immunoprecipitated with ab186745 at 1/50 dilution (Lane1). Lane 2: PBS instead of PC-3 cell lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab186745 has been referenced in 7 publications.
- Carpio MA et al. BOK controls apoptosis by Ca2+ transfer through ER-mitochondrial contact sites. Cell Rep 34:108827 (2021). PubMed: 33691099
- Li YL et al. Sildenafil improves right ventricular remodelling in monocrotaline-induced rats by decreasing myocardial apoptosis and activating peroxisome proliferator-activated receptors. J Pharm Pharmacol 73:145-151 (2021). PubMed: 33793805
- Okazaki J et al. MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer. Digestion 101:794-806 (2020). PubMed: 31563901
- Muenchow A et al. The BCL-2 selective inhibitor ABT-199 sensitizes soft tissue sarcomas to proteasome inhibition by a concerted mechanism requiring BAX and NOXA. Cell Death Dis 11:701 (2020). PubMed: 32839432
- Zheng JH et al. Intrinsic Instability of BOK Enables Membrane Permeabilization in Apoptosis. Cell Rep 23:2083-2094.e6 (2018). PubMed: 29768206
- Stehle D et al. Contribution of BH3-domain and Transmembrane-domain to the Activity and Interaction of the Pore-forming Bcl-2 Proteins Bok, Bak, and Bax. Sci Rep 8:12434 (2018). PubMed: 30127460
- Jelinek M et al. Differing Mechanisms of Death Induction by Fluorinated Taxane SB-T-12854 in Breast Cancer Cells. Anticancer Res 37:1581-1590 (2017). PubMed: 28373418