If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab58825 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
I recently purchased an antibody: Mouse monoclonal to bone sialoprotein (ab58825). The product page says to check the for the lot specific reconstitution volume. However, when I go to the page where usually I have entered the lot there is no option to do so. My lot number is 843351. Can you please advise me as to the reconstitution volume appropriate for this lot? |
|||||||||
ANSWER: |
Thank you for your enquiry. I sincerely apologize for the confusing statement on the datasheet. Please rest assured that this has been removed. For reconstitution, please add 100ul of distilled water to the vial to make up a concentration of 1 mg/ml. I hope this information will be helpful. If there is anything else that I can help you with, please do not hesitate to contact me. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-Bone Sialoprotein antibody [ID1.2] (ab58825) at 1 µg/ml + MG-63 Human Osteosarcoma Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Bone Sialoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
0
Call 01223 696 000 or contact us