![Western blot - Bone Sialoprotein antibody [ID1.2] (ab58825) image](#Bone-Sialoprotein-Primary-antibodies-ab58825-4.jpg)
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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Overview
Product name
Anti-Bone Sialoprotein antibody [ID1.2]
See all Bone Sialoprotein products (5) ...
Description
Mouse monoclonal [ID1.2] to Bone Sialoprotein
Specificity
ab58825 recognizes the ~70-80 kDa bone sialoprotein. It does not cross-react with osteocalcin, osteonectin, osteopontin, or bone alkaline phosphatase.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified human Bone Sialoprotein
Positive control
Human osteoblasts or osteocytes
Properties
Form
Lyophilised:
Reconstitute with distilled water.
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.04% Thimerosal (merthiolate), <0.1% sodium azide
Constituents: 50mM Tris, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified IgG
Clonality
Monoclonal
Clone number
ID1.2
Isotype
IgG1
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Western blot - Bone Sialoprotein antibody [ID1.2] (ab58825)
![Western blot - Bone Sialoprotein antibody [ID1.2] (ab58825) image](/ps/datasheet/images/58/ab58825/Bone-Sialoprotein-Primary-antibodies-ab58825-4.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab58825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Post-translational
modifications
N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314.
Cellular localization
Secreted.
Target information above from: UniProt accessionP21815
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 2 antibody
- Bone sialoprotein 2 precursor antibody
see all
Database links
- Entrez Gene: 3381 Human
- Omim: 147563 Human
- SwissProt: P21815 Human
- Unigene: 518726 Human
Anti-Bone Sialoprotein antibody [ID1.2] images:
Western blot - Bone Sialoprotein antibody [ID1.2] (ab58825)
Anti-Bone Sialoprotein antibody [ID1.2] (ab58825) at 1 µg/ml + MG-63 Human Osteosarcoma Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Bone Sialoprotein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Bone Sialoprotein antibody [ID1.2] (ab58825)
ab58825 has not yet been referenced specifically in any publications.
Publishing research using ab58825? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"