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Product Name
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Bone Sialoprotein antibody [ID1.2]
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See all Bone Sialoprotein antibodies (5)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [ID1.2] to Bone Sialoprotein
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Immunogen
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Purified human Bone Sialoprotein
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Reacts with
(species key)
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Hu
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Specificity
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ab58825 recognizes the ~70-80 kDa bone sialoprotein. It does not cross-react with osteocalcin, osteonectin, osteopontin, or bone alkaline phosphatase.
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 35 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human osteoblasts or osteocytes
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Cellular localization
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Secreted
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Research areas
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Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Relevance
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The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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ID1.2
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.04% Thimerosal (merthiolate), <0.1% sodium azide
Constituents: 50mM Tris, pH 7.4 Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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Purified IgG
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Form
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Lyophilised
Reconstitute with distilled water. Please see label for lot-specific reconstitution volume.
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bone Sialoprotein antibody [ID1.2] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Bone Sialoprotein
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Bone Sialoprotein antibody [ID1.2] - more information
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Customer reviews (feedback) regarding Bone Sialoprotein antibody [ID1.2] |
Customer FAQs regarding Bone Sialoprotein antibody [ID1.2] |
Protocols for Bone Sialoprotein antibody [ID1.2] |
Price and availability of products related to Bone Sialoprotein antibody [ID1.2] |
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Bone Sialoprotein antibody [ID1.2] - other tools:
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