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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Overview
Product name
Anti-Bone Sialoprotein antibody
See all Bone Sialoprotein products (5) ...
Description
Rabbit polyclonal to Bone Sialoprotein
Tested applications
ELISA, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A region within synthetic peptide: SATTLGYGED ATPGTGYTGL AAIQLPKKAG DITNKATKEK ESDEEEEEEE, corresponding to N terminal amino acids 108-157 of Human Bone Sialoprotein
SATTLGYGED ATPGTGYTGL AAIQLPKKAG DITNKATKEK ESDEEEEEEE
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS.
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/62500ELISA: 1/62500
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 42 kDa (predicted molecular weight: 35 kDa).
IHC-P: Use a concentration of 5 µg/mlPerform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Post-translational
modifications
N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314.
Cellular localization
Secreted.
Target information above from: UniProt accessionP21815
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 2 antibody
- Bone sialoprotein 2 precursor antibody
see all
Database links
- Entrez Gene: 3381 Human
- Omim: 147563 Human
- SwissProt: P21815 Human
- Unigene: 518726 Human
Anti-Bone Sialoprotein antibody images:
Western blot - Bone Sialoprotein antibody (ab33022)
Anti-Bone Sialoprotein antibody (ab33022) at 1.25 µg/ml + HepG2 cell lysate
Secondary
HRP conjugated anti Rabbit IgG at 1/50000 dilution
Predicted band size : 35 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bone Sialoprotein antibody(ab33022)
IHC image of ab33022 staining in normal human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33022, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Bone Sialoprotein antibody (ab33022)
ab33022 has not yet been referenced specifically in any publications.
Publishing research using ab33022? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"