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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
Overview
Product name
Anti-Bone Sialoprotein antibody
See all Bone Sialoprotein products (5) ...
Description
Rabbit polyclonal to Bone Sialoprotein
Specificity
ab52128 does not cross react with human Osteonectin, or human Osteopontin.
Tested applications
ELISA, RIA, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Human Bone Sialoprotein (bone extract)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bone Sialoprotein antibody(ab52128)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa.
IHC-P: Use a concentration of 2 µg/ml
ICC/IF
ICC/IF: 1/100ICC/IF: 1/100
Target
Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Post-translational
modifications
N-glycosylated; glycans consist of sialated and core-fucosylated bi-, tri- and tetraantennary chains.
O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.
Sulfated on either Tyr-313 or Tyr-314.
Cellular localization
Secreted.
Target information above from: UniProt accessionP21815
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BNSP antibody
- Bone sialoprotein 2 antibody
- Bone sialoprotein II antibody
see all
Database links
- Entrez Gene: 3381 Human
- Entrez Gene: 24477 Rat
- Omim: 147563 Human
- SwissProt: P21815 Human
- SwissProt: P13839 Rat
- Unigene: 518726 Human
- Unigene: 9721 Rat
Anti-Bone Sialoprotein antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Bone Sialoprotein antibody(ab52128)
ab52128 (2ug/ml) staining Bone Sialoprotein in human bone using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of Bone sialoprotein in sectretory compartments in the cells of the perichondrium
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-Bone Sialoprotein antibody (ab52128)
ab52128 has not yet been referenced specifically in any publications.
Publishing research using ab52128? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"