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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Sialoprotein antibody (ab78278)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Sialoprotein antibody (ab78278)
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Product Name
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Bone Sialoprotein antibody
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See all Bone Sialoprotein antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Bone Sialoprotein
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Immunogen
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Purified full length Human native protein
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Reacts with
(species key)
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Hu, Rat
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Specificity
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Does not cross react with Human osteopontin or osteonectin by RIA or ELISA.
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Tested applications
(see key)
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ELISA, IHC-P, RIA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: 1/2500 at 2-8°C o/n in moist chamber.
RIA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 35 kDa (Note: the protein can be heavily glycosylated).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human fetal tibia or calvaria.
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Cellular localization
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Secreted
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Research areas
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Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Bone
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Relevance
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The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Storage buffer
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Preservative: None
Constituents: Whole serum
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Bone Sialoprotein antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Bone Sialoprotein antibody images, references, products related to ab78278 and other tools.
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Bone Sialoprotein antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Sialoprotein antibody (ab78278)
Immunohistochemistry analysis of paraffin-embedded Human fetal calvaria bone tissue using 1/2500 ab78278 o/n at 4°C and a biotinylated secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Bone Sialoprotein antibody (ab78278)
Immunohistochemistry analysis of paraffin-embedded Human fetal calvaria bone tissue using 1/2500 ab78278 o/n at 4°C and a biotinylated secondary antibody.
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Search PubMed (MEDLINE) for references to Bone Sialoprotein
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Bone Sialoprotein antibody - more information
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Customer reviews (feedback) regarding Bone Sialoprotein antibody |
Customer FAQs regarding Bone Sialoprotein antibody |
Protocols for Bone Sialoprotein antibody |
Price and availability of products related to Bone Sialoprotein antibody |
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Bone Sialoprotein antibody - related products:
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Bone Sialoprotein antibody - other tools:
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Contact Abcam with a Technical Enquiry about Bone Sialoprotein antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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