Anti-Bordetella pertussis IgA Human ELISA Kit (ab108708)
- Product nameAnti-Bordetella pertussis IgA Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Pos. Serum 20 7.1% Pos. Serum 24 4.9%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s anti-Bordetella pertussis IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Bordetella pertussis in Human serum and plasma.
A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgA sample captured in plate.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Bordetella pertussis (IgA) Coated Microplate (12 x 8 wells) 1 unit Bordetella pertussis anti-IgA HRP Conjugate Black cap 1 x 20ml Bordetella pertussis IgA Cut-off Control Green cap 1 x 3ml Bordetella pertussis IgA Negative Control Blue cap 1 x 2ml Bordetella pertussis IgA Positive Control Red cap 1 x 2ml Cover foil 1 unit IgA Sample Diluent White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Research Areas
Our Abpromise guarantee covers the use of ab108708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Anti-Bordetella pertussis IgA Human ELISA Kit (ab108708)
ab108708 has not yet been referenced specifically in any publications.