Anti-Bordetella pertussis IgA Human ELISA Kit (ab108708)

Overview

  • Product nameAnti-Bordetella pertussis IgA Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Pos. Serum 12 5.18%
    Pos. Serum 12 6.94%
  • Tests
    1 x 96 test
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Bordetella pertussis IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Bordetella pertussis in Human serum and plasma.

     

    A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Bordetella pertussis-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Bordetella pertussis IgA sample captured in plate.

  • Tested applicationsIndirect ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108708 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for Anti-Bordetella pertussis IgA Human ELISA Kit (ab108708)

    ab108708 has not yet been referenced specifically in any publications.

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