Anti-Bordetella pertussis toxin antibody [20.6] (ab27158)
Key features and details
- Mouse monoclonal [20.6] to Bordetella pertussis toxin/ptxD
- Suitable for: ELISA, WB
- Reacts with: Bordetella pertussis
- Isotype: IgG1
Overview
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Product name
Anti-Bordetella pertussis toxin antibody [20.6]
See all Bordetella pertussis toxin/ptxD primary antibodies -
Description
Mouse monoclonal [20.6] to Bordetella pertussis toxin/ptxD -
Host species
Mouse -
Specificity
Reacts with the intact toxin as well as the S2 subunit. Some reactivity towards the toxoid is also present. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Bordetella pertussis -
Immunogen
Synthetic peptide corresponding to Bordetella pertussis toxin/ptxD.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
20.6 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/100000.
|
|
WB |
1/1000.
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Notes |
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ELISA
1/100000. |
WB
1/1000. |
Target
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Relevance
Pertussis toxin contains five different chains, S1-S5. They are organized into 2 functional subunits: A, composed of S1 (which is toxic) and B, containing S2, S3, S5, and two copies of S4 (B binds to the membrane receptors). Dimers of S2-S4 and S3-S4 are held together by S5 -
Database links
- Entrez Gene: 2665068 Bordetella pertussis
- SwissProt: P04977 Bordetella pertussis
- SwissProt: P04978 Bordetella pertussis
- SwissProt: P04979 Bordetella pertussis
- SwissProt: P04981 Bordetella pertussis
- SwissProt: P0A3R5 Bordetella pertussis
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Alternative names
- IAP S1 antibody
- Islet-activating protein S1 antibody
- NAD-dependent ADP-ribosyltransferase antibody
Datasheets and documents
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Datasheet download
References (1)
ab27158 has been referenced in 1 publication.
- Ibsen PH The effect of formaldehyde, hydrogen peroxide and genetic detoxification of pertussis toxin on epitope recognition by murine monoclonal antibodies. Vaccine 14:359-68 (1996). PubMed: 8735545