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Read our guarantee »Overview
Product name
Bovine Heart Mitochondrial lysate
Tested applications
SDS-PAGE, WBmore details
Properties
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Constituent: 99% Iso-osmotic Buffer
Concentration
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Lysate notes
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.
Applications
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Our Abpromise guarantee covers the use of ab110338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
References for Bovine Heart Mitochondrial lysate (ab110338)
This product has been referenced in:
- Shell JR & Lawrence DS Proteolytic regulation of the mitochondrial cAMP-dependent protein kinase. Biochemistry 51:2258-64 (2012).Read more (PubMed: 22385295) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"