Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)
Key features and details
- Mouse monoclonal [1H10] to Bovine Papilloma Virus E2
- Suitable for: WB, ICC/IF
- Reacts with: Bovine papillomavirus
- Isotype: IgG
Overview
-
Product name
Anti-Bovine Papilloma Virus E2 antibody [1H10]
See all Bovine Papilloma Virus E2 primary antibodies -
Description
Mouse monoclonal [1H10] to Bovine Papilloma Virus E2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Bovine papillomavirus -
Immunogen
Recombinant full length Bovine Papillomavirus E2-protein expressed in E Coli.
-
Epitope
Mapped to amino acids 208-218 (RPDGVWVASEG) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1H10 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa.
WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
|
ICC/IF |
Use a concentration of 1 µg/ml.
1/1000 for 1 hour at RT, in PBS with 1% BSA. |
Notes |
---|
WB
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa. WB: 1/1000 - 1/5000 for 1 hour at RT, in TBST with 2% non-fat dry milk. |
ICC/IF
Use a concentration of 1 µg/ml. 1/1000 for 1 hour at RT, in PBS with 1% BSA. |
Target
-
Relevance
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
Cellular localization
Host cell nucleus -
Database links
- SwissProt: P03122 Bovine papillomavirus
-
Alternative names
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
Images
-
All lanes : Anti-Bovine Papilloma Virus E2 antibody [1H10] (ab77174)
Lane 1 : Recombinant purified Bovine Papillomavirus E2 protein at 10000 µg
Lane 2 : Recombinant purified Bovine Papillomavirus E2 protein at 5000 µg
Lane 3 : Recombinant purified Bovine Papillomavirus E2 protein at 1000 µg
Lane 4 : Recombinant purified Bovine Papillomavirus E2 protein at 500 µg
Predicted band size: 45 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab77174 has been referenced in 2 publications.
- Kurg R et al. Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers. J Virol 80:11218-25 (2006). WB . PubMed: 16943289
- Kurg R et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol 73:4670-7 (1999). WB, ELISA . PubMed: 10233926