Anti-Bovine Papilloma Virus E2 antibody [5H4] (ab1072)
Key features and details
- Mouse monoclonal [5H4] to Bovine Papilloma Virus E2
- Suitable for: ICC/IF, ELISA, IP
- Reacts with: Bovine papillomavirus
- Isotype: IgG1
Overview
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Product name
Anti-Bovine Papilloma Virus E2 antibody [5H4]
See all Bovine Papilloma Virus E2 primary antibodies -
Description
Mouse monoclonal [5H4] to Bovine Papilloma Virus E2 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, ELISA, IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Bovine papillomavirus -
Immunogen
Recombinant full length protein corresponding to Bovine Bovine Papilloma Virus E2. Recombinant full length BPV type 1 E2 protein purified from E. coli
Database link: P03122 -
Epitope
Conformational -
Positive control
- ICC/IF: Human U2OS cells expressing BPV E2 protein
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5H4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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ELISA |
Use at an assay dependent dilution.
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IP |
Use at an assay dependent dilution.
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Notes |
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ICC/IF
Use a concentration of 1 - 2 µg/ml. |
ELISA
Use at an assay dependent dilution. |
IP
Use at an assay dependent dilution. |
Target
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Relevance
The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication. -
Cellular localization
Host cell nucleus -
Database links
- SwissProt: P03122 Bovine papillomavirus
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Alternative names
- BPVgp4 antibody
- E2 antibody
- Regulatory protein E2 antibody
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab1072 has been referenced in 1 publication.
- Boehm TMSA et al. A case series of canine cutaneous inverted papilloma with one case showing evidence of recurrence. Vet Dermatol 32:268-e74 (2021). PubMed: 33908122