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Anti-Bovine Serum Albumin antibody [1C12] (ab79827)

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Overview

Product name

Anti-Bovine Serum Albumin antibody [1C12]
See all Bovine Serum Albumin products (12) ...

Description

Mouse monoclonal [1C12] to Bovine Serum Albumin

Specificity

This antibody reacts specifically with BSA. It does not cross react with Human Serum Albumin or Albumin from other species.

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Cow

Does not react with

Rabbit, Human

Immunogen

Bovine Serum Albumin.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

1C12

Isotype

IgG1

Light chain type

kappa

Research areas

Cardiovascular >> Blood >> Serum Proteins

  • Western blot - Bovine Serum Albumin antibody [1C12] (ab79827)Western blot - Bovine Serum Albumin antibody [1C12] (ab79827) image (enlarge)

  • ELISA - Bovine Serum Albumin antibody [1C12] (ab79827)ELISA - Bovine Serum Albumin antibody [1C12] (ab79827) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab79827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Cellular localization

Secreted

Alternative names

  • ALB antibody
  • Albumin antibody
  • Allergen Bos d 6 antibody
  • BSA antibody
  • Serum albumin antibody
see all

Database links

Anti-Bovine Serum Albumin antibody [1C12] images:

  Western blot - Bovine Serum Albumin antibody [1C12] (ab79827)

Western blot - Bovine Serum Albumin antibody [1C12] (ab79827)

All lanes : Anti-Bovine Serum Albumin antibody [1C12] (ab79827) at 1/2000 dilution

Lane 1 : Human Serum
Lane 2 : Bovine Serum
Lane 3 : Rabbit Serum

Lysates/proteins at 10 µl per lane.

Secondary
Goat anti-mouse IgG (H+L) peroxidase conjugate at 1/4000 dilution

Predicted band size : 69 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)


Exposure time : 1 minute

Non-denaturing SDS-PAGE (7.5%).

  ELISA - Bovine Serum Albumin antibody [1C12] (ab79827)

ELISA - Bovine Serum Albumin antibody [1C12] (ab79827)

Graph showing comparison of the reactivity of ab79287 with Bovine Serum Albumin, Ovalbumin, Rabbit Serum Albumin and Human Serum Albumin.

References for Anti-Bovine Serum Albumin antibody [1C12] (ab79827)

ab79827 has not yet been referenced specifically in any publications.

Publishing research using ab79827? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"