Anti-Bovine Serum Albumin antibody [5F9] (ab9092)

Anti BSA antibodies, polyclonal, unconjugated

ANSWER:

Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

http://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
http://www.abcam.com/abreviews

Customer would like to know if this antibody was purified from cell culture or ascites.

ANSWER:

Ab9092 was purified from ascites.

Was the BSA antibody (mouse monoclonal [5F9] to Bovine Serum Albumin) derived from ascites or tissue culture?

ANSWER:

Thank you for your enquiry. We would like to confirm that this antibody derived from ascites.

I am sorry to say that now I have got a new problem with your antibody ab9092. I have tried to show that I don´t have any bovine serum left in a cell culture, just human serum, with no success.Last week I tested diluted human serum at the same time as diluted bovine serum in a Western blot, both got positive. In your product data sheet it says that the antibody should not cross react with human albumin. Now when it does I can´t use it for the purpose it was meant. I have already bought it twice, because I trusted your information and didn´t check the cross reactivity until now. What shall we do about it?

ANSWER:

Albumin is the most abundant protein in serum. I don't know what dilutions you did to human serum? The antibody would non-specifically bind to HSA when HSA overwhelmingly presented in the loading sample. I suggest you try to dilute both sera at least 1:1000 and titer down the antibody too.

I have used your antibody ab9092 in western blot with no success. The gel and blotting are done with methods I normally use. Purified BSA have been included as positive control. The secondary antibody was a rabbit anti- mouse-Ig HRP-conjugated which has worked in other blotting assays. Do you have any idea what is wrong?

As blocking buffer I use PBS with 1% Tween 20 and as dilution buffer PBS with 0.05% Tween 20. This is my ordinary protocol for Western blot. Best regards,

ANSWER:

Thank you for your message. We would suggest trying PBS with 0.1% Tween 20 and 1.5% fish gel as blocking and dilution buffer. Load 20uL of purified BSA at 0.5mg/mL and use 1:1000 dilution of the primary antibody.