![Western blot - Bovine Serum Albumin antibody [5F9] (ab9092) image](#ab9092_1.gif)
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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Bovine Serum Albumin antibody [5F9]
See all Bovine Serum Albumin products (12) ...
Description
Mouse monoclonal [5F9] to Bovine Serum Albumin
Specificity
This antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Cow
Does not react with
Chicken, Human
General notes
This product contains 0.02% (w/v) Sodium Azide preservative.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M Potassium Phosphate, 0.15M sodium chloride, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5F9
Myeloma
Sp2/0
Isotype
IgG1
Light chain type
unknown
Research areas
-
Western blot - Bovine Serum Albumin antibody [5F9] (ab9092)
![Western blot - Bovine Serum Albumin antibody [5F9] (ab9092) image](/ps/datasheet/Images/9/ab9092/ab9092_1.gif)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab9092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
This product was assayed by immunoblot using IRDye800TM Goat-anti-Mouse IgG.
A working dilution of 1:5,000 is suggested for this assay.
Researchers should determine optimal titers for other applications.
The antibody may be suitable for other applications, although no supporting data is available
ELISA: Use at an assay dependent dilution.
Target
Relevance
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
Cellular localization
Secreted
Alternative names
- ALB antibody
- Albumin antibody
- Allergen Bos d 6 antibody
see all
Database links
Anti-Bovine Serum Albumin antibody [5F9] images:
Western blot - Bovine Serum Albumin antibody [5F9] (ab9092)
Anti-Bovine Serum Albumin is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2). In both lanes 20 ul of sample was applied. Primary antibody is diluted 1:5000 in PBS and is reacted for 1.5 h at room temperature.
Detection occurs using a 1:5000 dilution of IRDye800TM Conjugated Goat-a-Mouse IgG reacted for 1 h at room temperature. Visualization is by near-infrared (IR) western blotting analysis using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR Biosciences, Lincoln, NE.
References for Anti-Bovine Serum Albumin antibody [5F9] (ab9092)
This product has been referenced in:
- Li Xet al. Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran. Colloids Surf B Biointerfaces 61:113-7 (2008).Read more (PubMed: 17703925) »
See 1 publication for this product
Publishing research using ab9092? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"