Anti-Bovine Serum Albumin antibody [BSA-7G10] (ab3781)

Anti BSA antibodies, polyclonal, unconjugated

ANSWER:

Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

http://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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sandwich ELISA with ab3781 and ab7637
using BSA
problem: no signal
sandwich ELISA is untested for both antibodies

send testing discount code for trying in sELISA

ANSWER:

Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately, our guarantee does not apply in this case.
Please let me know the other 2 anti-BSA antibodies that you would like to try together in sandwich ELISA and I'd be happy to issue you the testing discount codes as well.
DISCOUNT CODE: xxx for ab3781
DISCOUNT CODE: xxx for ab7637
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab3781 and ab7637 in sandwich ELISA. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Dear technical support: This customer raised an enquiry about ab79827 (Anti-Bovine Serum Albumin antibody [1C12]) and ab3781 (Anti-Bovine Serum Albumin antibody [BSA-7G10]) He would likes to know the concentration and storage buffer of these antibodies. Could you please help this customer to solve these problems? Thanks for your kindly assistance.

ANSWER:

Thank you for contacting us.

These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply and produce fluid (ascites) in its abdomen. This fluid contains a high concentration of antibody which can be harvested. This usually provides higher antibody yields than hybridoma cell culture. Unfortunately, the concentration of the specific antibody cannot be determined as there are many proteins present in ascites. Furthermore, these antibodies are not further purified from the fluid harvested and thus no particular storage buffer is added.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

I am using your product ab19195 and ab3781.I am going to see the antibody (ab3781) load on to a nanofibre film. I have done ELISA but here i am going to use A 1cm*1cm nanofer film instead of ELISA plate, so would please help me how I can do this and measure the color change after adding substrate?is there any toll free no I can contact you?please let me know.

ANSWER:

Thank you for contacting us. Feel free to call us any time between 8am and 8pm EST at 888-77-ABCAM.

I am not sure what you mean by detecting the antibody on a nanofiber film. Is this film a matrix for growing cells? If so, you could use an ICC staining protocol such as the one linked below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11417

Alternatively, is the nanofiber film similar to a WB membrane? In that case, you could use a Dot Blot protocol:

http://www.abcam.com/index.html?pageconfig=resource&rid=11452

I hope this helps, please let me know if you need any additional information.

I am interested in a monoclonal antibody for BSA, I would like to know if you could provide the epitope to which BSA is binding, if we purchase this antibody (ab3781).

ANSWER:

Thank you for your enquiry.

Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region.

I am sorry I can't be of more assistance in this occasion but if there is anything else that I can help you with, please do not hesitate to contact me.