Anti-Brachyury / Bry antibody [1H9A2] (ab140661)
Key features and details
- Mouse monoclonal [1H9A2] to Brachyury / Bry
- Suitable for: WB, IHC-P, Flow Cyt, ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Brachyury / Bry antibody [1H9A2]
See all Brachyury / Bry primary antibodies -
Description
Mouse monoclonal [1H9A2] to Brachyury / Bry -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant Human Brachyury /Bry protein; HEK293 cells transfected with recombinant Human Brachyury / Bry fragment (aa 257-309); HeLa cells; Jurkat and Raji cell lysates; Human cervical cancer and rectum cancer tissues.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR136145-8 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1H9A2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 47 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. -
Involvement in disease
Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6862 Human
- Omim: 601397 Human
- SwissProt: O15178 Human
- Unigene: 389457 Human
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Alternative names
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.
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All lanes : Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : HEK293 cell lysate, transfected with recombinant Human Brachyury / Bry fragment (aa 257-309)
Predicted band size: 47 kDa -
Flow cytometric analysis of HeLa cells labeling Brachyury / Bry with ab140661 at 1/200 dilution (green); negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling Brachyury / Bry with ab140661 at 1/200 dilution followed by DAB staining.
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Anti-Brachyury / Bry antibody [1H9A2] (ab140661) at 1/500 dilution + Recombinant Human Brachyury / Bry protein
Predicted band size: 47 kDa
Expected MW is 31.2 kDa. -
ELISA analysis using ab140661. Antigen = Purified recombinant fragment, corresponding to amino acids 257-309 of Human Brachyury / Bry, expressed in E. Coli.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab140661 has been referenced in 4 publications.
- Hwang JW et al. iPSC-Derived Embryoid Bodies as Models of c-Met-Mutated Hereditary Papillary Renal Cell Carcinoma. Int J Mol Sci 20:N/A (2019). PubMed: 31575031
- Otani R et al. Brachyury gene copy number gain and activation of the PI3K/Akt pathway: association with upregulation of oncogenic Brachyury expression in skull base chordoma. J Neurosurg 128:1428-1437 (2018). PubMed: 28753115
- Liu Q et al. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960 (2018). PubMed: 30154523
- Jezkova J et al. Brachyury identifies a class of enteroendocrine cells in normal human intestinal crypts and colorectal cancer. Oncotarget N/A:N/A (2016). WB, IHC . PubMed: 26862851