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Western blot - Brachyury / Bry antibody (ab57480)
Western blot - Brachyury / Bry antibody (ab57480)
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Product Name 

Brachyury / Bry antibody

 

See all Brachyury / Bry antibodies (2)...

Product type 

Primary antibodies

Description 

Mouse monoclonal to Brachyury / Bry

Immunogen 

Recombinant fragment: ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL SMLQSHDNW, corresponding to amino acids 222-321 of Human Brachyury / Bry
BLAST 'ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Lineage specification >> Mesoderm
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Stem Cells >> Lineage Markers >> Mesoderm

Relevance 

This transcription factor is the earliest indicator of mesoderm formation. Used as a marker of mesodermal differentiation, Brachyury is expressed during gastrula and neurula stages in involuting mesoderm and in the notochord.

 

Alternative names Brachyury / Bry antibody (ab57480)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

6862    (Human)

Omim

    

601397    (Human)

SwissProt

    

O15178    (Human)

Raised in 

Mouse

Clonality 

Monoclonal

Isotype 

IgG2a

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: None
PBS, pH 7.2

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Brachyury / Bry antibody is on this datasheet. But please do contact us if you would like any reassurance!


 

Brachyury / Bry antibody images:

Product image  Western blot - Brachyury / Bry antibody (ab57480)

Western blot - Brachyury / Bry antibody (ab57480)



Predicted band size : 47 kDa


Brachyury / Bry antibody (ab57480) at 1ug/lane + A-549 cell lysate at 25ug/lane.

 
 

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