Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Stem Cells >> Lineage Markers >> Mesoderm
Overview
Product name
Anti-Brachyury / Bry antibody
See all Brachyury / Bry products (2) ...
Description
Mouse monoclonal to Brachyury / Bry
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment: ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL SMLQSHDNW, corresponding to amino acids 222-321 of Human Brachyury / Bry
ERSDHKEMME EPGDSQQPGY SQWGWLLPGT STLCPPANPH PQFGGALSLP STHSCDRYPT LRSHRSSPYP SPYAHRNNSP TYSDNSPACL SMLQSHDNW
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
-
Western blot - Brachyury / Bry antibody (ab57480)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab57480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1-5 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
Involvement in disease
Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
T is involved in susceptibility to the development of chordoma (CHDM) [MIM:215400]. Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Note=Susceptibility to development of chordomas is due to a T gene duplication.
Sequence similarities
Contains 1 T-box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15178
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BRAC_HUMAN antibody
- Brachyury homolog antibody
- Brachyury protein antibody
see all
Database links
- Entrez Gene: 6862 Human
- Omim: 601397 Human
- SwissProt: O15178 Human
- Unigene: 389457 Human
Anti-Brachyury / Bry antibody images:
Western blot - Brachyury / Bry antibody (ab57480)
Predicted band size : 47 kDa
Brachyury / Bry antibody (ab57480) at 1ug/lane + A-549 cell lysate at 25ug/lane.
References for Anti-Brachyury / Bry antibody (ab57480)
ab57480 has not yet been referenced specifically in any publications.
Publishing research using ab57480? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"