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Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

Q&A (10)Specific References (3)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab29466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References for Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

This product has been referenced in:
  • Moore SF  et al. Dual regulation of glycogen synthase kinase 3 (GSK3)a/ß by protein kinase C (PKC)a and Akt promotes thrombin-mediated integrin aIIbß3 activation and granule secretion in platelets. J Biol Chem 288:3918-28 (2013). WB . Read more (PubMed: 23239877) »
  • Soyal SM  et al. A greatly extended PPARGC1A genomic locus encodes several new brain-specific isoforms and influences Huntington disease age of onset. Hum Mol Genet 21:3461-73 (2012). Read more (PubMed: 22589246) »

See all 3 Publications for this product

Publishing research using ab29466 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab29466

Displaying 1 - 10 of 10 results for Q&A

10
Votes: Highest First

Does the lysate have phosphatase inhibitors in it?

Thank you very much for having patience.

I had tried to call the laboratory last Friday, unfortunately they haven't had the information handy - they agreed to send me an email as early as possible.. I am sorry for the delay I will get this confir...

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I would like to try your antibody cat no. ab125280 but I would like to have a positive control. I found your normal human brain tissue lysate (cat no. ab29466). I just want to make sure that with that antibody, using your lysate as a sample I am suppose t...

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Ab125280 has been tested with human brain tissue samples, so ab29466 is a good positive control to use with this antibody. According to UniProt, DCC is expressed in axons in the central nervous system, so the human brain tissue should definitely...

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Dear technical support: This customer has highly interested in ab29466 (Brain (Human) Tissue Lysate - adult normal tissue) and ab29468 (Brain (Human) Whole Cell Lysate - liver cirrhosis), he would like to know the currently in stock lot number of these ly...

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Thank you for contacting us.

For ab29466, the current lot number is XXXXX containing 100 ug. It belongs to a 26 years old male.

For ab29468, the lot number available is XXXXX also containing 100ug. It belongs to a 87 years old male. Read More

Hi Sir/Madam,
Good evening.
I had received the human heart (Ab 29431 Lot: GR8337-3) and brain (Ab 29466 Lot: GR62059-2) lysates end of April at the condition of 4oC and tested the lysates with 1D SDS-PAGE recently.
1 ug of lysate loaded in ...

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Thank you for taking thetime to contacting us and report the problems you have been experiencing with the tissue lysates ab29431 and ab29466. I am sorry to hear that they have not been of the quality youwere expecting.

The details provided will...

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ab58803 gave no bands in WB using positive control ab3871
ab39973 gave no bands in WB using positive control ab29466

Thank you for contacting us.

As we discussed on the phone, you may be able to improve your WB results by switching from milk to BSA and by using a higher concentration of primary antibody (1:500 for example). If you are unable to improve your re...

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I'm currently interested in buying from Abcam human brain lysates samples to check protein expression levels by WB. However, before ordering I would like to know whether in the membrane fraction samples post synaptic density proteins/ cytoskeletal as...

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Thank you for contacting us. The lab sourcing the lysates informed me that they never measured any post synaptice density proteins/ cytoskeletal associated proteins, but only a membrane protein marker Na/K ATPase in the membrane fractions, so they ca...

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We ordered this product three times. The first shipment was good. The last two are as shown as western image.

Thanks for confirming that information. I am sending a free of charge vial of ab29466 on the order ***. If this vial is acceptable, I'd be happy to send another since you ordered two vials that weren't suitable. Alternatively I could issue a credit or ref...

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The main problem we had with your 'normal brain lysate' is the fact that our protein of interest is expressed at very different levels when we compare one vial of your 'normal brain lysate' to another. If you could send us another vial to compare/replace ...

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Thank you for your reply. I am checking on the region of the brain from which the lysate is prepared, and I would be happy to send another vial of ab29466. Could you tell me the PO#, order#, or date that the vial was ordered on? I see a few orders...

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Beta actin levels were low. The protein concentration must be low.

Thanks for your call today and for letting us know about the trouble with ab29466. As we discussed, please send an image of the Western blot results using ab29466 and a protocol. I look forward to receiving this information and resolving th...

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I'm using the brain tissue lysate (ab29466) for western blotting, and I'd like to know which brain regions (cortex, hippocampus, amygdala etc.) are included in the lysate because this information is critical for further analysis.　Sorry for taking your tim...

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Thank you for your enquiry. Unfortunately, there is no information on which brain region was used to make this tissue lysate from the vendor. Therefore, the product was catagorized as brain tissue lysate in general. Should you wish to purchase tissue ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"