Brain (Mouse) Whole Cell Lysate - normal tissue, 14 days old (ab7189)

Overview

  • Product nameBrain (Mouse) Whole Cell Lysate - normal tissue, 14 days old
    See all Plasma lysates
  • General notesSource: Outbred NIH-Swiss Albino mice (14 days old)
  • Tested applicationsWBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 5% Beta mercaptoethanol, 12.5% Glycerol, 1% SDS, 1% Triton-X-100, 1% Sodium Deoxycholate, 0.01% Bromophenol blue, 5µg/ml Leupeptin, 5µg/ml Aprotinin, 50mM Tris HCl, 150mM Sodium chloride, 1mM EDTA, 1mM PMSF, pH 6.8
  • Concentration information loading...
  • Lysate notes The mouse brain tissue was collected from fourteen (14) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab7189 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB Use at an assay dependent dilution. 10 µg to 20 µg per lane is recommended for mini gel.

    References for Brain (Mouse) Whole Cell Lysate - normal tissue, 14 days old (ab7189)

    ab7189 has not yet been referenced specifically in any publications.

    Product Wall

    Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 1155271 und sollte morgen bei Ihnen ankommen.

    Das Molekulargewicht kann man folgendermaßen errechnen: Öffnen Sie die SwissProt Lin...

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    Vielen Dank fürIhre Anfrage.

    Da das Lysat bei uns vorrätig ist, kann es am nähsten Tag geliefert werden. Wenn Sie mir also die Lieferadresse mitteilen, kann ich den Versandt gleich abwickeln. Es sollte übrigens kein Probl...

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    Vielen Dank für Ihren Anruf und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen.



    Ich möchte folgende Bemerkungen zu Ihrem Protokoll machen:

    1. Ich denke, Sie detektieren das richtige Protei...

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    Thank you for contacting us. I presume you are looking for positive control used in western blot. Our customers have used this ab successfully with mouse brain tissue and 293 cells lysates. Please check the Abreviews. The Mouse brain lysates ca...

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    Thank you for your enquiry. We do not produce 100ug at a time, but many milligrams from multiple mouse brains. The exact number can vary with each batch. The brain has a lot of protein, so it may only take one mouse brain to get 100ug. I hope t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"