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Branched Chain Amino Acid Assay Kit (ab83374)

Q&A (3)

Overview

  • Product nameBranched Chain Amino Acid Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids
  • Assay typeQuantitative
  • Sensitivity
    > 0.2 nmol/well
  • Assay time
    0h 40m
  • Product overview

    Abcam's Branched Chain Amino Acid Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). Abcam's Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceThe branched-chain amino acids or BCAA’s, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA’s are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA’s, primarily Leu, can stimulate insulin secretion. The BCAA’s have also been implicated in a wide range of other physiological effects.
    • Alternative names
        BCAAIsoleucineLeucineValine

    Applications

    Our Abpromise guarantee covers the use of ab83374 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Branched Chain Amino Acid Assay Kit images

    • Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)
      Functional Studies - Branched Chain Amino Acid Assay Kit (ab83374)
      Leucine Standard Curve performed according to the attached protocol.

    Protocols

    References for Branched Chain Amino Acid Assay Kit (ab83374)

    ab83374 has not yet been referenced specifically in any publications.

    Publishing research using ab83374 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab83374

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    Inquiry: Branched Chain Amino Acid Assay Kit (ab83374) Does this kit work with only single amino acids? Or is it also active with di/tripeptides?

    Thank you for contacting us.

    This kit will detect all branched chain amino acids, regardless of whether they are single amino acids or peptides.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need ...

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    Hello,

    Do you have any informations for testing muscle homogenate samples with your kits ab65347 and ab83374 ?
    Thanks in advance for your help
    --

    Merci de nous avoir contactés.

    Le laboratoire m'a confirmé que nous n'avons malheureusement pas de données expérimentales pour ces kits sur des lysats musculaires.

    Désolé de ne pas pouvoir vous a...

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    Hallo,

    My client have a some questions about Branched chain amino acid assay kit # ab83374

    Does using this kit can be determined the sum of branched chain amino acids (Leu + Ile + Val) ? Whether only leucine (standard contains the sa...

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    Thank you for your inquiry.

    I am happy to confirm that this kit will measure all the Branched chain amino acids (BCAAs).

    All BCAAs will be deaminated to produce NADH, which is what this kit will actually measure.
    The Leucine in th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"