Branched Chain Amino Acid Assay Kit (ab83374)
- Product nameBranched Chain Amino Acid Assay Kit
- Tests100 x 1 assay
- Sample typeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids
- Assay typeQuantitative
- Sensitivity> 0.2 nmol/well
- Assay time0h 40m
- Product overview
Abcam's Branched Chain Amino Acid Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (λmax = 450 nm). Abcam's Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
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The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests BCAA Assay Buffer WM 1 x 25ml BCAA Enzyme Mix (lyophilized) Green 1 vial Leu Standard (1 µmol) Yellow 1 x 10µl WST Substrate Mix (lyophilized) Red 1 vial
- Research Areas
Our Abpromise guarantee covers the use of ab83374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Branched Chain Amino Acid Assay Kit images
Leucine Standard Curve performed according to the attached protocol.
References for Branched Chain Amino Acid Assay Kit (ab83374)
ab83374 has not yet been referenced specifically in any publications.