RabMAb

Anti-Brd4 antibody [EPR5150(2)] (ab128874)

Overview

  • Product nameAnti-Brd4 antibody [EPR5150(2)]
    See all Brd4 primary antibodies
  • Description
    Rabbit monoclonal [EPR5150(2)] to Brd4
  • Tested applicationsWB, IP, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Brd4.

  • Positive control
    • HeLa, Caco2, T.T, RAW264.7 and NIH3T3 cell lysates; Human colon carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab128874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).
IP Use at 1 µg/mg of lysate.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionPlays a role in a process governing chromosomal dynamics during mitosis.
    • Tissue specificityUbiquitously expressed.
    • Involvement in diseaseNote=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
    • Sequence similaritiesContains 2 bromo domains.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Brd4 antibody
      • BRD4_HUMAN antibody
      • Bromodomain containing 4 antibody
      • bromodomain containing protein 4 antibody
      • Bromodomain-containing protein 4 antibody
      • CAP antibody
      • chromosome associated protein antibody
      • HUNK1 antibody
      • HUNKI antibody
      • MCAP antibody
      • Protein HUNK1 antibody
      see all

    Anti-Brd4 antibody [EPR5150(2)] images

    • All lanes : Anti-Brd4 antibody [EPR5150(2)] (ab128874) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Caco2 cell lysate
      Lane 3 : T.T. cell lysate
      Lane 4 : RAW264.7 cell lysate
      Lane 5 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 152 kDa
    • ab128874 at 1/100 dilution staining Brd4 in Human colon carcinoma tissue by Immunohistochemistry.
    • ab128874 (1/500) staining Brd4 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counter stained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

      See Abreview

    • ab128874 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/ml in 50 mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 (1/10000) was used to confirm successful immunoprecipation.

      See Abreview

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Brd4 antibody [EPR5150(2)] (ab128874)

    ab128874 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunoprecipitation
    Immuno-precipitation step Protein G
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Total protein in input 1000 µg
    Username

    Abcam user community

    Verified customer

    Submitted Apr 25 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
    Loading amount 15 µg
    Specification mouse embryonic fibroblasts
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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    Abcam user community

    Verified customer

    Submitted Jan 23 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (pancreatic tumor)
    Loading amount 25 µg
    Specification pancreatic tumor
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (pancreatic cancer cell line)
    Loading amount 25 µg
    Specification pancreatic cancer cell line
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Oct 19 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (endometrial adenocarcinoma)
    Specification endometrial adenocarcinoma
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization Yes - PBST
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Abcam user community

    Verified customer

    Submitted Oct 08 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (endometrial adenocarcinoma)
    Specification endometrial adenocarcinoma
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization Yes - PBST
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 08 2012

    Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding ab128874.

    The immunogen is a peptide taken from between amino acids 175 and 200 of human Brd4.

    I hope this informati...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"