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Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Brd4.
(Peptide available as ab85811.)
Our Abpromise guarantee covers the use of ab75898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at 1 µg/mg of lysate.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).Can be blocked with Brd4 peptide (ab85811).|
ab75898 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 (1/10000) was used to confirm successful immunoprecipation.
Detects band around 150 kDa (predicted size) also additional band around 250 kDa (possibly modified Brd4).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"