• Product nameAnti-Brd4 antibodySee all Brd4 primary antibodies ...
  • Description
    Rabbit polyclonal to Brd4
  • Tested applicationsICC/IF, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Brd4.

    (Peptide available as ab85811.)

  • Positive control
    • This antibody gave a positive signal in Human, Mouse and Rat Liver Tissue Lysates.



Our Abpromise guarantee covers the use of ab75898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).Can be blocked with Brd4 peptide (ab85811).


  • FunctionPlays a role in a process governing chromosomal dynamics during mitosis.
  • Tissue specificityUbiquitously expressed.
  • Involvement in diseaseNote=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • Sequence similaritiesContains 2 bromo domains.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession O60885 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Brd4 antibody
    • BRD4_HUMAN antibody
    • Bromodomain containing 4 antibody
    • bromodomain containing protein 4 antibody
    • Bromodomain-containing protein 4 antibody
    • CAP antibody
    • chromosome associated protein antibody
    • HUNK1 antibody
    • HUNKI antibody
    • MCAP antibody
    • Protein HUNK1 antibody
    see all

Anti-Brd4 antibody images

  • All lanes : Anti-Brd4 antibody (ab75898) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Liver (Rat) Tissue Lysate
    Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)

    Lysates/proteins at 10 µg/ml per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP)
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 152 kDa
    Observed band size : 171 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 150 seconds
  • ab75898 (1/500) staining Brd4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • ICC/IF image of ab75898 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75898, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 5µg/ml.

References for Anti-Brd4 antibody (ab75898)

This product has been referenced in:
  • Puissant A  et al. Targeting MYCN in neuroblastoma by BET bromodomain inhibition. Cancer Discov 3:308-23 (2013). WB ; Human . Read more (PubMed: 23430699) »
  • Bartholomeeusen K  et al. Bromodomain and Extra-terminal (BET) Bromodomain Inhibition Activate Transcription via Transient Release of Positive Transcription Elongation Factor b (P-TEFb) from 7SK Small Nuclear Ribonucleoprotein. J Biol Chem 287:36609-16 (2012). WB . Read more (PubMed: 22952229) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Loading amount 15 µg
Specification mouse embryonic fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 29 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Feb 03 2012

Thank you for your inquiry. I am very sorry to hear that you are having problem with this antibody. As I understand from your e-mail, there is some problem with co-IP. Unfortunately, as the datasheet indicates this product has been tested in IC...

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