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Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Brd4.
(Peptide available as ab85811.)
"Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help."
Our Abpromise guarantee covers the use of ab75898 in the following tested applications.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IP||Use at 1 µg/mg of lysate.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 152 kDa).|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Brd4 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: Hu brain whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab75898 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.