BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)

Overview

  • Product nameBrdU Cell Proliferation ELISA Kit (chemiluminescent)See all BrdU kits ...
  • Detection methodLuminescent
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeSandwich (qualitative)
  • Sensitivity
    < 10 cells/well
  • Assay time
    2h 00m
  • Assay durationMultiple steps standard assay
  • Product overview

    ab126572 involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.

    The horseradish peroxidase catalyzes the oxidation of diacylhydrazides. The reaction product, in its excited state, decays yielding light in the process. This reaction has a large dynamic range spanning many logs of relative luminescence. The intensity of the luminescence is proportional to the amount of incorporated BrdU in the cells. The reaction is quantified using a luminometer and is plotted as relative light units per second (RLU/s).

    The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.

  • Tested applicationsIndirect ELISAmore details
  • PlatformMicroplate

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 200 tests
    BrdU Reagent 1 x 15µl
    Conjugate Diluent 1 x 25ml
    Fixing Solution 1 x 40ml
    Peroxidase Goat anti-mouse IgG (2000X) 1 x 15µl
    Plate wash concentrate (50X) 1 x 200 tests
    Prediluted anti-BrdU detecting antibody 1 x 20ml
    Reaction Buffer 1 x 24ml
    Substrate 1 x 800µl
  • Research Areas

    Applications

    Our Abpromise guarantee covers the use of ab126572 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Indirect ELISA Use at an assay dependent concentration.

    Protocols

    References for BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)

    ab126572 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"