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BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)

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Overview

  • Product nameBrdU Cell Proliferation ELISA Kit (colorimetric)See all BrdU kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Sensitivity
    < 40 cells/well
  • Assay time
    2h 30m
  • Product overview

    ab126556 involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.

    The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stopping reagent), the intensity of which is proportional to the amount of incorporated BrdU in the cells. The colored reaction product is quantified using a spectrophotometer.

    The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.

  • Tested applicationsIndirect ELISA more details

Properties

Applications

Our Abpromise guarantee covers the use of ab126556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Indirect ELISA I-ELISA

BrdU Cell Proliferation ELISA Kit (colorimetric) images

  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of MCF7 cells per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of MCF7 cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of Jurkat cells (non-adherent) per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of Jurkat cells (non-adherent) per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of RH7777 (adherent) cells per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Indirect ELISA - BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    Detection of RH7777 (adherent) cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal -to-noise ratio.

Protocols

References for BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)

ab126556 has not yet been referenced specifically in any publications.

Publishing research using ab126556 ? Please let us know so that we can cite the reference in this datasheet

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The associated PO was: xxxxxxx

Thank you for the additional information. I have arranged for this replacement vial of Peroxidase Goat anti-mouse IgG (2000X) to be shipped. You should receive it by the end of next week. For your records, the order reference number associated with this r...

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I had centrifuged the vial at 10000g for five minutes, but still could not pick up any solution.

Thank you for your email and I apologize for the delay in my reply.

I have aquired a new vial of Peroxidase Goat anti-mouse IgG (2000X) that I'd like to send. So I can process this shipment, please provide the PO number or Abcam order reference ...

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I am contacting due to an issue with a Brdu cell proliferation ELIZA kit (ab126556) recently purchased from your company (lot GR76685-6). Unfortunately upon opening one of the vials (Peroxidase Goat anti-mouse IgG (2000X) which was supposed to contai...

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Thank you for your enquiry.

I just want to confirm that you have centrifuged the vial of Peroxidase Goat anti-mouse IgG (2000X) to ensure any droplets potentially on the sides and in the lid have been recovered.

Thanks in advance for t...

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