• Product nameBrdU Immunohistochemistry Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Product overview

    ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

    The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.

    Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956 


  • Notes

    *Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.



  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details



    Our Abpromise guarantee covers the use of ab125306 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr Use at an assay dependent concentration.
    IHC-P Use at an assay dependent concentration.

    BrdU Immunohistochemistry Kit images

    • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
    • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
    • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.


    References for BrdU Immunohistochemistry Kit (ab125306)

    This product has been referenced in:
    • Luo C  et al. Recombinant Reg3ß protein protects against streptozotocin-induced ß-cell damage and diabetes. Sci Rep 6:35640 (2016). Mouse . Read more (PubMed: 27767186) »
    • Ozeki N  et al. Interleukin-1ß-induced autophagy-related gene 5 regulates proliferation of embryonic stem cell-derived odontoblastic cells. PLoS One 10:e0124542 (2015). Mouse . Read more (PubMed: 25894570) »

    See all 3 Publications for this product

    Product Wall

    clean-grade SD rat was fasted from 12 h prior to the establishment of the pancreatitis model until 24 h after the model was established, but drinking water was made continuously available. The rat resumed regular intake of food and water thereafter. On day 7 after pancreatitis was induced, the rat was sacrificed by cervical dislocation. At 6 h before the rat was sacrificed and tissue samples were collected, BrdU was administered via IP injection at 100 mg/kg body weight.

    After 7 days of the establishment of pancreatitis, BrdU-positive cells formed cell sheets.

    Abcam user community

    Verified customer

    Submitted Aug 07 2015

    BrdU immunohistochemistry kit

    Poor Excellent 5/5 (Ease of Use)
    Very easy to use, however disappointing results (high non-specific background staining) when used exactly as per kit instructions. Poor compared to ab2284 (also shown in image) which was tested in parallel and which worked very nicely.

    Abcam user community

    Verified customer

    Submitted Aug 27 2014

    The detection antibody in ab125306 is an affinity purified sheep polyclonal conjugated to biotin. This antibody is not available for purchase separately outside of the kit.

    Following the staining protocol on page 11 of the protocol for ab125306, with frozen tissue section you would skip step 1 "Deparaffinization" and start with step 2.1. You will also skip step 2.2, as you do not need to perform antigen retrieva...

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    To answer your questions about ab125306:
    1) For suspension cells – is cytospin the only preparation method for suspension cells?
    It is possible to prepare slides by preparing a 'slurry' suspension of cells and air drying them onto slides. Fol...

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    Many thanks for having patience!

    The catalogue number we have is ab129956.


    I have contacted datasheet team for creating the new datasheet. I will let you know the price and catalogue number soon.

    Thank you for contacting us.

    After checking with lab, I can confirm that we can offer the control slides. These slides are with formalin fixed-paraffin embedded mouse intestine sections, pulsed with BrdU.

    Could you confirm if you a...

    Read More

    Thank you for contacting us.

    I can confirm that I have sent an email to my colleague in lab. I will get back to you with the answer soon.

    Many thanks for having patience!