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Overview

  • Product nameBrdU Immunohistochemistry Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Product overview

    ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.

    The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.

  • Notes

    *Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.

    Control Slides: Mouse intestinal tissue pulsed with BrdU.

  • Tested applicationsIHC-Fr, IHC-P more details

Properties

Applications

Our Abpromise guarantee covers the use of ab125306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr IHC-Fr
IHC-P IHC-P

BrdU Immunohistochemistry Kit images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)
    Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.

Protocols

References for BrdU Immunohistochemistry Kit (ab125306)

ab125306 has not yet been referenced specifically in any publications.

Publishing research using ab125306 ? Please let us know so that we can cite the reference in this datasheet

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I have some questions about this BrdU kit. Please see below.

To answer your questions about ab125306:
1) For suspension cells – is cytospin the only preparation method for suspension cells?
It is possible to prepare slides by preparing a 'slurry' suspension of cells and air drying them onto slides. Follow...

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Thank you for your reply. Could you please give me some idea of when the datasheet will be available and what will be the likely time for delivery following placing an order. It is important that I keep my client informed of timelines.

Many tha...

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Many thanks for having patience!

The catalogue number we have is ab129956.

Thanks!

That is great news. Yes we are still interested in buying them. Can you let me know the cost per slide, cat# etc I will need to order them.

Many thanks,

I have contacted datasheet team for creating the new datasheet. I will let you know the price and catalogue number soon.

Customer would like to buy the control slide?

Thank you for contacting us.

After checking with lab, I can confirm that we can offer the control slides. These slides are with formalin fixed-paraffin embedded mouse intestine sections, pulsed with BrdU.

Could you confirm if you are ...

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Customer would like to buy the control slide?

Thank you for contacting us.

I can confirm that I have sent an email to my colleague in lab. I will get back to you with the answer soon.

Many thanks for having patience!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"