BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)

Product Name 

BrdU antibody [BU1/75 (ICR1)] (HRP)

Product type 

Primary antibodies

Description 

Rat monoclonal [BU1/75 (ICR1)] to BrdU (HRP)

Immunogen 

The details for the immunogen of this antibody are not available.

Species Reactivity 

Not applicable.

Specificity 

This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-Fr, IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: 1/25 - 1/200.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody is also available in Unconjugated (ab6326), FITC (ab74545) and Biotin (ab74547) conjugated formats.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers

Relevance 

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.

BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

BU1/75 (ICR1)

Isotype 

IgG2a

Purity 

Protein G purified

Storage buffer 

Preservative: None
Constituents: 50% carb/biocarb/glycerol, 10mg/ml BSA

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Conjugation 

Horse Radish Peroxidase

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

References for BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)

  This product has been used in: (two most recent references)

Perez-Ruiz A et al. beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci 121:1373-82 (2008). PubMed: 18397993

Iulianella A et al. Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis. Development 135:729-41 (2008). PubMed: 18223201
If you publish research using ab74546 please let us know so that we can cite the reference on this datasheet.

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