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Anti-BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)

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Overview

Product name

Anti-BrdU antibody [BU1/75 (ICR1)] (HRP)
See all BrdU products (17) ...

Description

Rat monoclonal [BU1/75 (ICR1)] to BrdU (HRP)

Conjugation

HRP

Specificity

This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.

Tested applications

ELISA, ICC/IF, IHC-Fr, IHC-Pmore details

Cross reactivity

Not applicable.

Immunogen

The details for the immunogen of this antibody are not available.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

BU1/75 (ICR1)

Isotype

IgG2a

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/250 - 1/1000.

    ELISA: 1/250 - 1/1000.

  • ShowHide

    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent concentration.

  • ShowHide

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

  • ShowHide

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution.

Target

Relevance

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Cellular localization

Nuclear

Alternative names

  • Bromodeoxyuridine antibody
  • BUdr antibody

References for Anti-BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)

This product has been referenced in:

  • Zheng Cet al. Prevention of Radiation-Induced Salivary Hypofunction Following hKGF Gene Delivery to Murine Submandibular Glands. Clin Cancer Res 17:2842-51 (2011). IHC-P. Read more (PubMed: 21367751) »
  • Perez-Ruiz Aet al. beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci 121:1373-82 (2008).Read more (PubMed: 18397993) »

See all 3 publications for this product

Publishing research using ab74546? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"