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Product Name
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BrdU antibody [BU1/75 (ICR1)] (HRP)
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See all BrdU - Proliferation Marker antibodies (19)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [BU1/75 (ICR1)] to BrdU (HRP)
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Immunogen
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The details for the immunogen of this antibody are not available.
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Species Reactivity
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Not applicable.
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Specificity
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This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions Flow Cyt: Use at an assay dependent dilution. ICC/IF: 1/25 - 1/200. IHC-P: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
This antibody is also available in Unconjugated (ab6326), FITC (ab74545) and Biotin (ab74547) conjugated formats.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides Tags & Cell Markers >> Cell Type Markers >> Replication Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker Cell Biology >> Cell Cycle >> Markers
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Relevance
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The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
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BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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BU1/75 (ICR1)
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Isotype
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IgG2a
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Purity
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Protein G purified
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Storage buffer
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Preservative: None Constituents: 50% carb/biocarb/glycerol, 10mg/ml BSA Material safety datasheet (MSDS) for this product: Glycerol MSDS
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BrdU antibody [BU1/75 (ICR1)] (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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References for BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)
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This product has been used in: (two most recent references)
Perez-Ruiz A et al. beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci 121:1373-82 (2008). PubMed: 18397993
Iulianella A et al. Cux2 (Cutl2) integrates neural progenitor development with cell-cycle progression during spinal cord neurogenesis. Development 135:729-41 (2008). PubMed: 18223201
If you publish research using ab74546 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BrdU
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BrdU antibody [BU1/75 (ICR1)] (HRP) - more information
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