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Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-BrdU antibody [BU1/75 (ICR1)] (HRP)
See all BrdU products (17) ...
Description
Rat monoclonal [BU1/75 (ICR1)] to BrdU (HRP)
Conjugation
HRP
Specificity
This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
Tested applications
ELISA, ICC/IF, IHC-Fr, IHC-Pmore details
Cross reactivity
Not applicable.
Immunogen
The details for the immunogen of this antibody are not available.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
BU1/75 (ICR1)
Isotype
IgG2a
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/250 - 1/1000.ELISA: 1/250 - 1/1000.
ICC/IF: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
Target
Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Cellular localization
Nuclear
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
References for Anti-BrdU antibody [BU1/75 (ICR1)] (HRP) (ab74546)
This product has been referenced in:
- Zheng Cet al. Prevention of Radiation-Induced Salivary Hypofunction Following hKGF Gene Delivery to Murine Submandibular Glands. Clin Cancer Res 17:2842-51 (2011). IHC-P. Read more (PubMed: 21367751) »
- Perez-Ruiz Aet al. beta-Catenin promotes self-renewal of skeletal-muscle satellite cells. J Cell Sci 121:1373-82 (2008).Read more (PubMed: 18397993) »
See all 3 publications for this product
Publishing research using ab74546? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"