Anti-BrdU antibody [BU1/75 (ICR1)] (ab6326)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)

Paraffin-embedded sections of hippocampus dentate gyrus stained for  BrdU-positive cells. The method is an indirect immunohistochemical technique: the primary antibody is ab6326 diluted 1/100 in PBS and the secondary antibody is an Alexa 488-conjugated anti-rat made in goat diluted 1/200.

  Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)

ab6326 used in double labeling on mouse sciatic nerve tissue (5µm) sections. This picture was kindly submitted by Dr Ulrich Hengst as part of his review on this product.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)

ab6326 at 1/200 staining mouse E14.5 embryonic telencephalon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retireval step was performed. The tissue was then stained with the antibody for 12 hours. A Texas Red conjugated donkey anti-rat antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Bing Lang

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)

ab6326 staining mouse adult epidermis tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking in 10% serum for 1 hr at RT.  The primary antibody was diluted 1/200 and incubated with the sample for 24 hrs at 4°C.  A biotinylated goat anti-rat antibody was used as the secondary followed by a streptavidin-Cy3®.

This image is courtesy of an anonymous Abreview

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  Immunocytochemistry - BrdU antibody [BU1/75 (ICR1)] (ab6326)

ab6326 staining cultured cells of rat brain tissue by ICC.  The sample was PFA fixed and permeabilized in 1M HCl prior to blocking with 5% serum for 1 hour at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 25°C.  A biotinylated rabbit anti-rat IgG antibody, diluted 1/200, was used as the secondary.

This image is courtesy of an anonymous Abreview

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  Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)

ab6326 staining BrdU in mouse brain tissue sections by IHC-Fr (paraformaldehyde-fixed frozen sections). Tissue samples were fixed with paraformaldehyde; permeabilized win 0.3% Triton X-100 and blocked with 5% Serum for 2 hours at 4°C. Before permeabilization samples were pretreated with 2N HCl at RT for 30 min and washed 3 times. The sample was incubated with primary antibody (1/200) at 4°C for 12 hours. An Alexa Fluor^® 488-conjugated Goat polyclonal to rat IgG (1/500) was used as secondary antibody. BrdU staining shown in green and NewN staining showin in red.

This image is courtesy of an anonymous Abreview

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