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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/ab6326_1.jpg)
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Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/ab6326_2.jpg)
(enlarge)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/ab6326_6.jpg)
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Product Name
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BrdU antibody [BU1/75 (ICR1)]
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See all BrdU - Proliferation Marker antibodies (17)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [BU1/75 (ICR1)] to BrdU
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Immunogen
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The details of the immunogen for this antibody are not available.
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Species Reactivity
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Not applicable.
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Specificity
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This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine.
The antibody does not cross react with IdU.
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-FoFr, IHC-Fr, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/40.
ICC/IF: 1/250
IHC-P: 1/40. It is strongly recommended to use an amplification method such as ABC or PAP/APAAP to visualize staining.
IHC-Fr: 1/40 - 1/200. In addition, found to work at 1/400 (see PMID: 16670699).
Immunohistochemistry (PFA fixed): 1/40 (from PubMed:16373695).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody is also available in FITC (ab74545), HRP (ab74546) and Biotin (ab74547) conjugated formats.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers
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Relevance
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The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.
BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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BU1/75 (ICR1)
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Isotype
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IgG2a
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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The antibody recognises single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although this doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimise results.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BrdU antibody [BU1/75 (ICR1)] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for BrdU antibody [BU1/75 (ICR1)] images, references, products related to ab6326 and other tools.
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BrdU antibody [BU1/75 (ICR1)] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
Paraffin-embedded sections of hippocampus dentate gyrus stained for BrdU-positive cells. The method is an indirect immunohistochemical technique: the primary antibody is ab6326 diluted 1/100 in PBS and the secondary antibody is an Alexa 488-conjugated anti-rat made in goat diluted 1/200.
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Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
ab6326 used in double labeling on mouse sciatic nerve tissue (5µm) sections. This picture was kindly submitted by Dr Ulrich Hengst as part of his review on this product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
ab6326 at 1/200 staining mouse E14.5 embryonic telencephalon tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retireval step was performed. The tissue was then stained with the antibody for 12 hours. A Texas Red conjugated donkey anti-rat antibody was used as the secondary. This image is courtesy of an Abreview submitted by Dr Bing Lang
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/ab6326_7.jpg)
ab6326 staining mouse adult epidermis tissue sections by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking in 10% serum for 1 hr at RT. The primary antibody was diluted 1/200 and incubated with the sample for 24 hrs at 4°C. A biotinylated goat anti-rat antibody was used as the secondary followed by a streptavidin-Cy3®. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunocytochemistry - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/ab6326_8.jpg)
ab6326 staining cultured cells of rat brain tissue by ICC. The sample was PFA fixed and permeabilized in 1M HCl prior to blocking with 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 25°C. A biotinylated rabbit anti-rat IgG antibody, diluted 1/200, was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)
![Immunohistochemistry (Frozen sections) - BrdU antibody [BU1/75 (ICR1)] (ab6326)](/ps/datasheet/images/6/ab6326/BrdU-Primary-antibodies-ab6326-10.jpg)
ab6326 staining BrdU in mouse brain tissue sections by IHC-Fr (paraformaldehyde-fixed frozen sections). Tissue samples were fixed with paraformaldehyde; permeabilized win 0.3% Triton X-100 and blocked with 5% Serum for 2 hours at 4°C. Before permeabilization samples were pretreated with 2N HCl at RT for 30 min and washed 3 times. The sample was incubated with primary antibody (1/200) at 4°C for 12 hours. An Alexa Fluor® 488-conjugated Goat polyclonal to rat IgG (1/500) was used as secondary antibody. BrdU staining shown in green and NewN staining showin in red. This image is courtesy of an anonymous Abreview
See Abreview
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References for BrdU antibody [BU1/75 (ICR1)] (ab6326)
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This product has been used in: (two most recent references)
Hristova M et al. Activation and deactivation of periventricular white matter phagocytes during postnatal mouse development. Glia 58:11-28 (2010). IHC-FoFr. PubMed: 19544386
Jacques TS et al. Combinations of genetic mutations in the adult neural stem cell compartment determine brain tumour phenotypes. EMBO J 29:222-35 (2010). IHC-P. PubMed: 19927122
See all 111 publication references for this product.
If you publish research using ab6326 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to BrdU
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BrdU antibody [BU1/75 (ICR1)] - more information
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