Anti-BrdU antibody (Biotin) (ab2284)

One more question: May I put this antibody in -800C for storage?

Thank you,

ANSWER:

Thank you for your reply.

This antibody can be at -80C in aliquots for storage, and this may extend the life of the antibody. Either -20C or -80C will be fine.

Please let me know if you have any further questions, and I look forward to hearing from you about the results with the replacement antibody. Have a nice evening!

I use your protocol for BrdU staining. Please see attached file. I used your antibody, ab2284 on mice intestine tissue last November, I got beautiful staining. This month, we are doing another batch of mice and following exactly protocol as November’s. I did three run of staining to exclude any mistake I might have and still got no stain at all. Finally, I thought it was DAB problem and emailed you last week. After receiving new DAB, I repeat BrdU stain with a fresh aliquated antibody. Then I got faint stain - comparing with last November’s staining. At that time, I thought it was Streaptavidin problem and emailed you for that. Since I need result quickly, I run another run with my real slides on Monday with the antibody I thawed last Thursday (got result on Friday) and got no stain at all even with new DAB. This antibody was aliquated and stored at -200C since we received last September. This particular tube was thawed last Thursday and used only once. It was put back to -200C right after usage. This Monday was the second time to thaw and to use, and I did not get any staining, the only explanation is that the antibody got degraded.

I hope you agree with me or give me some suggestion. I also hope you could send me new antibody to see what I get.

ANSWER:

Thank you for your reply and forsending the additional information.

It does sound like the antibody was damaged during storage, so I am happy to send a replacement vial for you. This will be coming free of charge on the order ***, which should arrive tomorrow. Please note that we recommend storing this antibody in working aliquots, so that each aliquot is not frozen and thawed more than one time.

I apologize for the trouble with these products, but please keep me updated about how this replacement antibody works in your staining. Let me know if you have any questions or if there is anything else that we can do for you.

We want to use the biotinylated anti-BrdU antibody without a secondary antibody. I was wondering if you would suggest blocking the tissue with hydrogen peroxide or denaturing the DNA with HCl first?

ANSWER:

Thank you for your inquiry.

You will denature the DNA first and then carry on with the IHC staining protocol.

http://www.abcam.com/index.html?pageconfig=resource&rid=11533
http://www.abcam.com/assets/popups/popup_datasheet_protocols_printer_friendly.cfm?pid=1325&intAbId=2284

I hope this information helps. Please contact us with any other questions.

ab8955: IHC-FrFl-geeignet?

ANSWER:

Vielen Dank für Ihren wirklich sehr netten Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens nach wurde der Antikörper ab8955 bisher noch nicht in IHC-FrFl getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab8955 in IHC-FrFl testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC-FrFl.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes" Unter der folgenden URL können Sie mehr über unser Abreview System erfahren: http://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: http://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

I have a question. Do you know if somebody try to precipitate chromatin with BrdU antibody? I would greatly appriciated your help.thank you in advance,

ANSWER:

Thank you for your enquiry.

I managed to find some references that you might be interested in.

1) Hiraga,et al. 2005. DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae. Genes to Cells. 10, 297-309. (http://www.genestocellsonline.org/cgi/content/full/10/4/297)

2) Lin, et al. 2006. Inhibition of estradiol-induced mammary proliferation by dibenzoylmethane through the E2–ER–ERE-dependent pathway. Carcinogenesis. 27(1):131-136

I hope this information will be useful to you. Should you require any further information, please do not hesitate to contact me.