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Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-BrdU antibody (Biotin)
See all BrdU products (17) ...
Description
Sheep polyclonal to BrdU (Biotin)
Conjugation
Biotin
Specificity
The antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 µg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. This product titers out at 50 ng/mL. For best results, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at a concentration of 100 to 500 µg/mL.
Tested applications
IHC-P, IHC-Fr, IP, ICC/IF, ELISA, IHC-FoFrmore details
Immunogen
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.15M PBS, pH 7.5
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution. (Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.)
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution. (dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.)
IHC-FoFr: 1/2000(1/2000 (see Abreview).)
Target
Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Cellular localization
Nuclear
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Anti-BrdU antibody (Biotin) images:
Immunohistochemistry (Formalin/PFA fixed sections) - BrdU antibody (Biotin) (ab2284)
ab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.
This image is courtesy of an Abreview submitted by Dr Christoph Schwarzer
Immunocytochemistry/ Immunofluorescence - BrdU antibody (Biotin) (ab2284)
ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
This image is courtesy of an anonymous Abreview
Protocols
References for Anti-BrdU antibody (Biotin) (ab2284)
This product has been referenced in:
- Banerjee NSet al. Human papillomavirus (HPV) E7 induces prolonged G2 following S phase reentry in differentiated human keratinocytes. J Biol Chem 286:15473-82 (2011).Read more (PubMed: 21321122) »
- Xie Zet al. Zbtb20 is essential for the specification of CA1 field identity in the developing hippocampus. Proc Natl Acad Sci U S A 107:6510-5 (2010). IHC-P; Mouse.Read more (PubMed: 20308569) »
See all 9 publications for this product
Publishing research using ab2284? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"