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Anti-BrdU antibody [MoBu-1] - Proliferation Marker (ab8039)

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1 question for ab8039

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Question 1

Thursday 30-May-2002

Do all antibodies to BrdU demand denaturation of DNA i.e. antibodies only attach into single-stranded DNA? Thanks! Anna Rissanen

ANSWER:

  

Yes. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.

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