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Products:Cell Biology >> Cell Cycle >> Markers
Overview
Product name
Anti-BrdU antibody [MoBu-1] - Proliferation Marker
See all BrdU products (17) ...
Description
Mouse monoclonal [MoBu-1] to BrdU - Proliferation Marker
Specificity
This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. The reaction shows a clear, nuclear confined speckled pattern.
Tested applications
ICC, IHC-P, Flow Cytmore details
Immunogen
5-bromodeoxyuridine conjugated with hemocyanine (isolated from Helix pomatia).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Purification notes
>95 % (by PAGE).
Clonality
Monoclonal
Clone number
MoBu-1
Isotype
IgG1
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides
Tags & Cell Markers >> Cell Type Markers >> Replication
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Cell Biology >> Cell Cycle >> Markers
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 2 µg/ml
IHC-P: Use at an assay dependent concentration.
Flow Cyt: Use a concentration of 1 - 2 µg/ml.
Target
Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
Cellular localization
Nuclear
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
References for Anti-BrdU antibody [MoBu-1] - Proliferation Marker (ab8039)
This product has been referenced in:
- Gonzalez-Nunez Vet al. Characterization of drCol 15a1b: a novel component of the stem cell niche in the zebrafish retina. Stem Cells 28:1399-411 (2010). IHC-P.Read more (PubMed: 20549708) »
- Kranz Det al. BRCA1 and Tip60 determine the cellular response to ultraviolet irradiation through distinct pathways. J Cell Biol 182:197-213 (2008). Flow Cyt.Read more (PubMed: 18625847) »
See all 4 publications for this product
Publishing research using ab8039? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"