Anti-BrdU antibody - Proliferation Marker (ab1893)

Does this antibody recognize Edu?

ANSWER:

T
Thank you for your patience in this matter.



I have been trying to determine if any of our BrdU antibodies are only specific to BrdU or not and it seems that most of them have some sort of cross reactivity with either ErdU or IrdU.

I am sorry that I could not help you further in this matter, but please let me know if there is anything else I can help you with.

A customer has the following enquiry;

On the data sheet, it says that these two antibodies should be stored at -20'C which is what I have done. Interestingly ab1893 does not seem to freeze and is still in a liquid state. Is this normal? Will ab37385 freeze too?

ab1893

Lot #: GR10346-10
Abcam invoice number: 1294594B



AB37385
Lot #: GR64701

Abcam invoice number 1308185



Many thanks for your time!

Kind regards,

ANSWER:

Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also no glycerol in storage buffer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thanks for take a look over this inquiry. attached please find our answers to the questions you asked.  I hope it to be of assistance for you the provide us with good and helpful support. I look forward to your reply soon, and a possible replacement for this item expected. Best regard

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement (ab1893) with the order number XXXXXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Can you tell me if this product also detects iododeoxyuridine (IDU)? If so, what is the percentage of reactivity?

ANSWER:

Thank you for your enquiry.

Unfortunately this antibody has not been tested against IDU.

Does the antibody pick up free Brdu? Also, do you know how I can remove unincorporated Brdu...perhaps dialysis?

ANSWER:

Thank you for your enquiry and your interest in our products.

The Brdu antibody should recognize both incorporated and free Brdu. The free Brdu should wash away in the experiment.

I hope this information will be useful.