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Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21118958|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19332057|
ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.
ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.