Anti-BrdU antibody - Proliferation Marker (ab1893)

Overview

  • Product nameAnti-BrdU antibody - Proliferation Marker
    See all BrdU primary antibodies
  • Description
    Sheep polyclonal to BrdU - Proliferation Marker
  • Tested applicationsIHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • Immunogen

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

Properties

Applications

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

Target

  • RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localizationNuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody - Proliferation Marker images

  • ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

    See Abreview

  • ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-BrdU antibody - Proliferation Marker (ab1893)

This product has been referenced in:
  • Madrigal MP  et al. Mesencephalic origin of the rostral Substantia nigra pars reticulata. Brain Struct Funct 221:1403-12 (2016). Read more (PubMed: 25579066) »
  • Martel G  et al. Genetic Demonstration of a Role for Stathmin in Adult Hippocampal Neurogenesis, Spinogenesis, and NMDA Receptor-Dependent Memory. J Neurosci 36:1185-202 (2016). Read more (PubMed: 26818507) »

See all 47 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Ramos cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate pH 6
Permeabilization No
Specification Ramos cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 20°C
Fixative Formaldehyde
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Submitted Dec 25 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Specification Liver
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Aug 25 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample African Green Monkey Cultured Cells (COS7)
Specification COS7
Permeabilization Yes - 0.5% TritonX100
Fixative Paraformaldehyde
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Submitted Jan 27 2014

Thank you for contacting us.

Unfortunately we do not have any cross-reactivity data against FDU either.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your telephone call yesterday afternoon.

I have received some further information regarding the protocol for use with ab1893 BrdU antibody.

There are two protocols we are aware of that have been used with this antibo...

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Thank you for contacting us. Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight. I hope this helps, please let me know if you need any additional information or assistance.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (colon cancer)
Specification colon cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jun 07 2012

T
Thank you for your patience in this matter.



I have been trying to determine if any of our BrdU antibodies are only specific to BrdU or not and it seems that most of them have some sort of cross reactivity with either ErdU or ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Paraformaldehyde fixed mouse brain sections)
Specification Paraformaldehyde fixed mouse brain sections
Fixative No
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Apr 19 2012

Thank you for contacting us.

We have checked the instock vials and can confirm that the liquid is frozen at -20C. You may need to check the freezer temperature because the antibody buffer should get frozen at -20C.

There is also n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"