Overview
Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR)
The components of this product are:
ab15580 Rabbit polyclonal to Ki67 - Proliferation Marker 50µg Concentration: Lot specific Applications: Flow Cyt: 1/100 ICC/IF: 1/100 - 1/1000 IHC-P: 1/200 IHC-Fr: 1/100-1000 IHC-FoFr: Use at an assay dependent dilution IHC-FrFl: 1/50 WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 345, 395 kDa (predicted molecular weight: 359 kDa)
ab37438 Rabbit polyclonal to Estrogen Receptor alpha 50µg Concentration:1 mg/ml Applications: ICC/IF: Use at a concentration of 5 µg/ml IHC-P: Use at a dilution of 1/100 WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 68 kDa
ab2428 Rabbit polyclonal to ErbB 2 250µl Concentration: 0.2 mg/ml Applications: IHC-P: Use at an assay dependent dilution IHC-Fr: Use at an assay dependent dilution IP: Use at an assay dependent dilution WB: 1/200
ab26 Mouse monoclonal [PAb 240] to p53 50µg Concentration: Lot specific Applications: ELISA: 1/100 Flow Cyt: Use at an assay dependent dilution ICC/IF: Use at an assay dependent dilution IHC-P: 1/250 - 1/500 IHC-Fr: 1/250 - 1/500 IHC (Methanol fixed): 1/250 IP: Use at 10µg/mg of lysate WB: 1/1000 - 1/2000. Detects a band of approximately 53 kDa
ab2764 Mouse monoclonal [PR-AT 4.14] to Progesterone Receptor - ChIP Grade 50µg Concentration:1 mg/ml Applications: ChIP: Use at an assay dependent dilution. (PubMed: 19553525) ICC/IF: 1/500. Using T47D cells. IHC-P: Use at a concentration of 5 µg/ml. IHC-Fr: Use at a concentration of 5 µg/ml. Immunohistochemical staining of PR in rat uterus with this antibody yields intense nuclear staining. IP: Use at an assay dependent dilution. WB: Use at a concentration of 5 µg/ml. By Western blot, this antibody detects a 94 kDa and a 120 kDa protein representing PRA and PRB, respectively from rat uterine homogenate. Predicted molecular weight: 99 kDa. (PubMed: 16912069)
Storage Instructions Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Components
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- Anti-Ki67 antibody - Proliferation Marker (ab15580)
- Flow Cyt, ICC, ICC/IF, IHC - Wmt, IHC-FoFr, IHC-Fr, IHC-FrFl, IHC-P, WB
- Rabbit polyclonal (50µg )
- 0.8mg/ml
- Reacts with: Hu, Ms, Rat, ChHm, Cow, Dog, Hrs, InMtj, Mk, SHm
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- Anti-Estrogen Receptor alpha antibody (ab37438)
- ICC/IF, IHC-FoFr, IHC-P, WB
- Rabbit polyclonal (50µg )
- 0.5mg/ml
- Reacts with: Hu, Ms, Rat
- Predicted: Cat, Hrs, Pig, Xl
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- Anti-ErbB 2 antibody (ab2428)
- IHC-Fr, IHC-P, IP, WB
- Rabbit polyclonal (250µl )
- 0.2mg/ml
- Reacts with: Hu, Ms, Rat
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- Anti-Progesterone Receptor antibody [PR-AT 4.14] - ChIP Grade (ab2764)
- ChIP, ICC/IF, IHC-Fr, IHC-P, IP, WB
- Mouse monoclonal (50µg )
- 1mg/ml
- Reacts with: Hu, Ms, Rat, Cow, Rb, RMk
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- Anti-p53 antibody [PAb 240] (ab26)
- Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB
- Mouse monoclonal (50µg )
- 1mg/ml
- Reacts with: Hu, Ms, Rat, ChHm, Cow, Dog, Mk, SHm
Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)Filipe Martins and Kornelia Polyak, Dana Faber Cancer InstituteParaffin embedded HER2+ tumor (IDC31) sections incubated with ErbB2 (ab2428). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion and the HER2+ amplified breast cancer cell line BT474). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)Filipe Martins and Kornelia Polyak, Dana Faber Cancer InstituteParaffin embedded primary ER+ breast tumor sections incubated with Progesterone Receptor (ab2764). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5-10 min. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)Filipe Martins and Kornelia Polyak, Dana Faber Cancer InstituteParaffin embedded primary ER+ breast tumor sections incubated with Estrogen receptor alpha (ab37438). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 5 min. (This antibody has also been tested successfully on xenograft derived from ER+ pleural effusion). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)Filipe Martins and Kornelia Polyak, Dana Faber Cancer InstituteParaffin embedded primary ER- breast tumor sections incubated with p53 (ab26). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/50 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 10 min. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)Filipe Martins and Kornelia Polyak, Dana Faber Cancer InstituteParaffin embedded primary ER- breast tumor sections incubated with ki67 (ab15580). Antigen retrieval was carried out using Citrate buffer (pH 6). Blocking step was carried out with 10% goat serum. The primary antibody was diluted 1/100 in 5% goat serum/PBS and incubated at room temperature for 4 hours. A biotin-conjugated secondary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour at room temperature. The samples were stained with DAB for 2-3 min.
References for Breast Cancer Biomarker Panel (Ki67, ER alpha, ErbB2, p53, PgR) (ab104827)
This product has been referenced in:
- Ucar A et al. miR-212 and miR-132 are required for epithelial stromal interactions necessary for mouse mammary gland development. Nat Genet 42:1101-8 (2010). Read more (PubMed: 21057503) »
- Abraira VE et al. Vertebrate Lrig3-ErbB interactions occur in vitro but are unlikely to play a role in Lrig3-dependent inner ear morphogenesis. PLoS One 5:e8981 (2010). Read more (PubMed: 20126551) »
