Breast (Human) Tissue Lysate - Adenocarcinoma (ab42033)

Overview

• Product nameBreast (Human) Tissue Lysate - AdenocarcinomaSee all Breast lysates ...
• General notesTissue: Breast Location: Left breast. Diagnosis: Adenocarcinoma. Stage: IIIA T₃N₁M₀ Grade: III Sex: Female Age: 49 years Gross findings: Not recorded. Microscopic findings: Not recorded. Soluble protein fraction

Properties

• FormLiquid
• Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferConstituents: 0.25% Sodium Deoxycholate, 0.1% SDS, PBS, 1µg/ml Pepstatin A, 1µg/ml Leupeptin, 1µg/ml Aprotinin, 1mM PMSF, 1mM EDTA, 1mM Sodium fluoride, 1mM Sodium Orthovanadate, pH 7.4
  
  Please note that 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate, as a result the lysate may not be suitable for applications which require native protein in a non-denaturing buffer. The final Laemmli buffer concs are: 31.25mM Tris HCl, pH 6.8, 12.5% Glycerol, 1% SDS, 0.005% Bromophenol Blue, 25mM TCEP.
• Concentration information loading...
• Purification NotesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
• Lysate notes This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab42033: Extraction 1: soluble protein fraction ab42033 Human Breast Tumor Tissue lysate ab42032 Human Breast Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab42035 Human Breast Tumor Tissue lysate ab42034 Human Breast Normal Tissue lysate (matched)
• Research Areas
  • Kits/ Lysates/ Other
  • Lysates
  • Tissue Lysates
  • Human
  • Other