General notesTissue: Breast
Location: Left breast.
Diagnosis: Infiltrating ductal carcinoma.
Stage: IIIA T3N1M0
Age: 47 years
Gross findings: Tumor size 6 cm., ill demarcated.
Cut section firm and white.
Ipsilateral axillary lymph nodes examined: 10
Microscopic findings: The tumor is composed of proliferation of malignant epithelial cells. The epithelial cells grown in the enlarged ducts contained comedo-necroses. The tumor cells have irregular, basophilic nuclei and abundant cytoplasm. Nuclear chromatin is coarse with prominent
nuclei. Mitotic figures are abundant. Some areas show micro-invasions. The stroma is infiltrated by numerous inflammatory cells. The lymph nodes show metastasis (6/10).
Insoluble protein fraction.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40 Constituents: PBS, 0.77% DTT, 0.1% SDS, 30.03% Urea, 15.22% Thiourea, 100% Laemmli buffer Note: 20µl of 5X Laemmli buffer were added per 100µl of tissue lysate
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Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab43538:
Extraction 1: soluble protein fraction
ab43536 Human Breast Tumor Tissue lysate
ab43535 Human Breast Normal Tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab43538 Human Breast Tumor Tissue lysate
ab43537 Human Breast Normal Tissue lysate (matched)