Brucella IgG Human ELISA Kit (ab108712)
Overview
- Product nameBrucella IgG Human ELISA Kit
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Species reactivityReacts with: Human
- Product overview
ab108712 Brucella IgG Human ELISA Kit is intended for the qualitative determination of IgG class antibodies against Brucella in human serum or plasma (citrate).
Brucella is a small Gram-negative bacterium (0.4-0.8 µm in diameter and 0.4-3.0 µm in length) which is non-flagellated, and non-spore forming. They are named after the military doctor David Bruce, who on Malta in 1887 isolated the pathogens from the spleen of a soldier who died of undulating fever. Four species are pathogenic to human: Brucella abortus, Brucella melitensis, Brucella suis and Brucella canis. All four species are exciters of Brucellosis, a disease characterized by undulating fever. Depending on exciter the disease is also called Morbus Bang (B. abortus) or Malta fever (B. melitensis).
The pathogens are transmitted from animals, which are mainly affected. The infection is caused by contact with ill animals or their excrements as well as by non-pasteurized milk and milk products like fresh cheese from sheep or goat. Main entrances are skin wounds, conjunctives and digestive tract. The intact pathogens are transported by granulocytes into local lymph nodes, from where they spread haematogenous. All kind of organs can be infected.
- Tested applicationsIndirect ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests Brucella anti-IgG Conjugate Blue, Black cap 1 x 20ml Brucella Coated Wells (IgG) 12 Strips of 8 wells 1 unit Brucella IgG Cut-off Control Yellow, Green cap 1 x 3ml Brucella IgG Negative Control Yellow, Blue cap 1 x 2ml Brucella IgG Positive Control Yellow, Red cap 1 x 2ml Cover foil 1 unit IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Washing Solution (20x conc.) White cap 1 x 50ml - Research Areas
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Alternative names
- B. abortus IgGB. canis IgGB. melitensis IgG
- B. suis IgGBrucella abortus IgGBrucella canis IgGBrucella melitensis IgGBrucella suis IgGMalta fever IgGMorbus Bang IgG
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Applications
Our Abpromise guarantee covers the use of ab108712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Indirect ELISA | I-ELISA |
Protocols
References for Brucella IgG Human ELISA Kit (ab108712)
ab108712 has not yet been referenced specifically in any publications.
