Anti-Brucella IgM Human ELISA Kit (ab108713)
- Product nameAnti-Brucella IgM Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Pos.Serum 24 3.9% Pos.Serum 24 5.7%
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeIndirect
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s anti-Brucella IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Brucella in Human serum and plasma.
A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgM sample captured in plate.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Brucella (IgM) Coated Microplate (12 x 8 wells) 1 unit Brucella anti-IgM HRP Conjugate 1 x 20ml Brucella IgM Cut-off Control 1 x 3ml Brucella IgM Negative Control 1 x 2ml Brucella IgM Positive Control 1 x 2ml Cover foil 1 unit IgM Sample Diluent 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Research Areas
- B. abortus IgG
- B. canis IgG
- B. melitensis IgG
- B. suis IgG
- Brucella abortus IgG
- Brucella canis IgG
- Brucella melitensis IgG
- Brucella suis IgG
- Malta fever IgG
- Morbus Bang IgG
Our Abpromise guarantee covers the use of ab108713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Anti-Brucella IgM Human ELISA Kit (ab108713)
ab108713 has not yet been referenced specifically in any publications.