Overview

  • Product nameAnti-Brucella IgM Human ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeIndirect
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-Brucella IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Brucella in Human serum and plasma.

     

    A 96-well plate has been precoated with Brucella antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Brucella-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Brucella IgM sample captured in plate.

  • Tested applicationsIndirect ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • B. abortus IgG
    • B. canis IgG
    • B. melitensis IgG
    • B. suis IgG
    • Brucella abortus IgG
    • Brucella canis IgG
    • Brucella melitensis IgG
    • Brucella suis IgG
    • Malta fever IgG
    • Morbus Bang IgG
    see all

Applications

Our Abpromise guarantee covers the use of ab108713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Protocols

References for Anti-Brucella IgM Human ELISA Kit (ab108713)

ab108713 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"