Overview

  • Product nameAnti-BubR1 antibody
    See all BubR1 primary antibodies
  • Description
    Mouse monoclonal to BubR1
  • Tested applicationsWB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-130 of Human BubR1

Properties

Applications

Our Abpromise guarantee covers the use of ab54894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 120 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionEssential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
  • Tissue specificityHighly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
  • Involvement in diseaseNote=Defects in BUB1B are associated with tumor formation.
    Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
    Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • DomainThe D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
    The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • Post-translational
    modifications
    Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
    Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
    Ubiquitinated. Degradated by the proteasome.
    Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
    Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
  • Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta homolg of S. cerevisiae BUB 1 antibody
    • Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
    • BUB 1B antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
    • Bub1A antibody
    • BUB1B antibody
    • BUB1B_HUMAN antibody
    • BUB1beta antibody
    • BUBR1 antibody
    • Budding Uninhibited by Benzimidazoles 1 beta antibody
    • Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody
    • hBUBR1 antibody
    • MAD3/BUB1 related protein kinase antibody
    • MAD3/BUB1-related protein kinase antibody
    • MAD3L antibody
    • Mitotic checkpoint gene BUB1B antibody
    • Mitotic checkpoint kinase MAD3L antibody
    • Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody
    • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
    • MVA1 antibody
    • OTTHUMP00000160319 antibody
    • Protein SSK1 antibody
    • SSK 1 antibody
    • SSK1 antibody
    see all

Anti-BubR1 antibody images

  • BubR1 antibody (ab54894) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.


  • Predicted band size : 120 kDa
    BubR1 antibody (ab54894) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab54894 staining BubR1 in murine neuronal progenior cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol, blocked using 0.01% BSA for 30 minutes and then incubated with ab54894 at a 1/100 dilution for 1 hour at 37°C. The secondary used was a rhodamine conjugated mouse monoclonal used at a 1/200 dilution.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab54894 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54894, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunofluorescence of nocodazole treated HeLa cells with ab54894 staining BubR1 (green). CREST was used to mark centromeres (red) and the DNA is stained with DAPI (blue).

    See Abreview

References for Anti-BubR1 antibody (ab54894)

This product has been referenced in:
  • Yong KJ  et al. 212Pb-radioimmunotherapy potentiates paclitaxel-induced cell killing efficacy by perturbing the mitotic spindle checkpoint. Br J Cancer 108:2013-20 (2013). Read more (PubMed: 23632482) »
  • Genin A  et al. Kinetochore KMN network gene CASC5 mutated in primary microcephaly. Hum Mol Genet : (2012). Human . Read more (PubMed: 22983954) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.1% for 10 min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Ms. Veronica Krenn

Verified customer

Submitted Jun 30 2012

Thank you for contacting us.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products. All of our products are covered by our Abpromise guarantee and if theantibodydoes not work as expected w...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neuronal progenitor cells)
Specification neuronal progenitor cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.01%
Username

Dr. Anna Kaplan Tzuk Ramon

Verified customer

Submitted Jan 12 2012

DISCOUNT CODE: 41XXXXX Expiration date: 18 April 2012 I am very pleased to hear you would like to accept our offer and test ab54894 in mouse ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, ...

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Thank you for your enquiry regarding ab54894. This antibody (ab54894) has been characterized in WB, IHC-P on human samples. Thus, testing it in ICC on mouse samples would be two untested/unknown factors (new species and application). Could you pleas...

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